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Epic Code LAB1230888 Trichomonas PCR

Important Note

Spectrum Health Regional Laboratory's Trichomonas testing is not validated for performance on pediatric patients under the age of 14 years old.  Testing for these patients must be collected in either an APTIMA Multi-Test Swab collection kit or the APTIMA Urine collection kit and will be sent out to a reference laboratory.  Please order a Reference Miscellaneous Test (#LAB848) and indicate complete test name when pediatric testing is necessary.

Test Name Alias

Trich | STI


Interface Order Alias


Clinical Information

Testing is performed using the FDA approved Abbott Alinity m STI Assay for the qualitative detection of ribosomal RNA (rRNA) from Trichomonas vaginalis (TV) to aid in the diagnosis of trichomoniasis in symptomatic and asymptomatic individuals. This assay may be used to test the following specimens collected with the Alinity m multi-Collect Specimen Collection Kit: vaginal swabs, endocervical swabs, female urine and male urine. Results for TV will be reported as Positive or Negative (presumed negative for Trichomonas vaginalis rRNA). For specimens receiving a result of Invalid, a new specimen should be collected.

Icons & Photos

SH/SHMG: Workday Order ITM-ITM-1190747

Non-SH: Supply form "STD Multi-Collection Kit"

Collection Instructions

Specimen Collection: Swab or Urine


Container(s): Alinity m Multi-Collect tube


Specimen Type: Female Genital Swab Alinity m Multi-Collect tube


  1. Specimens should be obtained with a swab using a speculum moistened with sterile warm water to facilitate collection.
  2. Lubricants other than water should not be used since they can be toxic to the organism.
  3. Discard disposable transfer pipette; it is not required for endocervical swab specimen collection
  4. Remove the sterile swab from the wrapper, taking care not to touch swab tip or lay it down on any surface
  5. Insert the white tip of the specimen collection swab into the endocervix canal.
  6. Gently rotate the swab for 15 – 30 seconds to ensure adequate sampling
  7. Withdraw the swab, carefully
  8. Handle the cap and tube carefully to avoid contamination, including the outside of the transport tube and cap. If necessary, change gloves.
  9. Unscrew the transport tube cap and immediately place the specimen collection swab into the transport tube so that the white tip is down.
  10. Carefully break the swab at the scored line on the shaft; use care to avoid splashing the contents
  11. Recap the transport tube. Ensure the cap seals tightly.
  12. Label the transport tube with sample ID, including date and time of collection.
  13. Transport to lab within 24 hours at room temperature or 14 days refrigerated.


Specimen Type: Male Genital Swab Alinity m Multi-Collect tube


  1. The patient must be instructed not to urinate at least 1 hour prior to sampling.
  2. Insert swab 2-4 cm into Urethra.
  3. Rotate swab for 3-5 seconds and withdraw swab.
  4. Insert swab into the Abbott multi-collect transport media. Snap off shaft of swab at the score line.
  5. Leave swab in tube. Cap tube and label specimen.
  6. Transport specimen within 24 hours at room temperature or 14 days refrigerated.


Specimen Type: Urine (First void)


Container(s): Sterile urine cup (with lid) or Alinity m multicollect sample kit

Preferred Volume to Collect: 30.0 mL
Minimum Volume to Collect: 10 mL if sterile cup is transported to lab; to fill line of Alinity m multicollect tube if transferred in office.

Aliquot: Yes - Transfer urine to Alinity m multicollect tube in office is preferred.

Collection Instructions:

  1. First void ("dirty") urine is included in this collection, this is collected before a UA specimen, if both are needed.
  2. Do not cleanse the genital area prior to specimen collection.
  3. Patient should not have urinated for at least one hour prior to sample collection.
  4. Follow directions above to obtain a first void urine specimen.
  5. Discard specimen collection swab, it is not required for urine specimen collection.
  6. Unscrew the transport tube cap, taking care not to spill the transport buffer within.
  7. Handle the cap and tube carefully to avoid contamination.
  8. Use the plastic transfer pipette to transfer urine from the collection cup into the transport tube until the liquid level in the tube falls within the clear fill window of the transport tube label or else a new specimen should be collected.
  9. Do not overfill. Slightly more than one full squeeze of the transfer pipette bulb may be required to transfer the necessary volume of urine specimen.
  10. Recap the transport tube carefully. Ensure the cap seals tightly.
  11. Label the transport tube with sample identification information, including date of collection. Take care not to obscure the fill window on the transport tube.


Transport Temperature:  Ambient (room temperature) or Refrigerated


NOTE:  Failure to collect a urine specimen, including the first part of the voiding, may decrease the sensitivity of this test. If the specimen cannot be delivered to the laboratory within a few hours after collection it should be stored and refrigerated until it can be delivered. Specimen must be received in laboratory for analysis within 24 hours of collection.


Specimen Type: Patient Self-Collect Swab

Self-collected vaginal swabs may be used as opposed to provider-collected swabs. Self-collected swabs are supported by current clinical guidelines as recent studies have shown their equivalence, if not superiority, in quality and their association with increased patient satisfaction.

NOTE: Self-collection must still take place in a healthcare setting and is not approved for at-home collection.

LINK: Self-Swab for Testing

LINK: References


Rejection Criteria

  • Leaky specimen
  • QNS
  • Urines in sterile cup not received into lab within 24 hours.

Specimen Stability

For Genital swabs:

  • Ambient: 14 days 2oC -30oC in Alinity m multi-collect  specimen container
  • Refrigerate: 14 days 2oC – 30oC in Alinity m multi-collect specimen container
  • Laboratory Retention: 7 days


For Urine:
If the urine is put directly in the Alinity m collection tube, the following applies:

  • Ambient: 14 days 2oC – 30oC in Alinity m multi-collect  specimen container
  • Refrigerate: 14 days 2oC – 30oC  in Alinity m multi-collect  specimen container
  • Laboratory Retention: 7 days

If only a urine cup is received

  • Refrigerate: 24 hours
  • Laboratory Retention: 24 hours

Test Frequency

Available Monday through Friday.

Usual TAT 1-3 days

Reference Range


Performing Department

Molecular Diagnostics

Performing Department Laboratory Location

Corewell Health Advanced Technology Laboratory (ATL), Grand Rapids, MI


Polymerase chain reaction (PCR)



CDM Code


Epic Test ID




Mayo Access Code

Current unavailable

Reviewed Date