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Epic Code LAB1230912 Vitamin D 1,25

Additional Codes

Beaumont Code: LAB5844

Interface Order Alias


Clinical Information

1,25-Dihydroxy vitamin D is the active form of vitamin D and is produced primarily in the kidney. Its measurement may be useful in the:

  • Assessment of vitamin D status in patients with significant kidney disease.
  • Differential diagnosis of hypercalcemia when the cause is not clear.
  • As part of the workup to evaluate a patient for possible metabolic bone disease.
  • Investigation of selected patients with vitamin D deficiency when the cause is unclear and/or inherited disorder is suspected (e.g., end-organ resistance to 1,25-dihydroxy vitamin D.

This test should NOT be used as an initial screen for vitamin D deficiency nor to assess vitamin D toxicity. Vitamin D, 25 Hydroxy is the appropriate test for initial assessment of vitamin D stores.

Collection Instructions

Container(s): Gold top (Serum Separator SST) 

Preferred Volume to Collect: 5 mL

Minimum Volume to Collect: 2 mL

Submission Container/Tube:  Plastic vial

Collection Instructions:

  • After collection, gently invert tube 8-10 times.
  • See Processing Instructions below.


PROCESSING INSTRUCTIONS (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Serum

Centrifuge/Spin: Yes

Aliquot: Yes

Processing Instructions:

  • Allow blood to clot for 30 minutes in a vertical position and centrifuge promptly.
  • (+S/PO/R)

Specimen Transport Temperature


Rejection Criteria

  • Plasma specimens.
  • Severely lipemic, icteric, or grossly hemolyzed specimens. 

Specimen Stability

Serum Specimens (Pour-Overs)

Ambient: 72 hours

Refrigerate: 7 days

Frozen: 6 months

Test Frequency

Twice a week.
Results available within 7 days.

Reference Range

19.9 – 79.3 pg/mL.

Performing Department


Performing Department Laboratory Location

Beaumont Royal Oak Special Testing Laboratory


Chemiluminescence Immunoassay (CLIA).



CDM Code


Epic Test ID




Reviewed Date