Sign in →

Epic Code LAB1230913 Alkaline Phosphatase Isoenzymes

Additional Codes

Beaumont Code: LAB5846

Interface Order Alias


Clinical Information

This assay is used to differentiate between liver, bone and intestinal isoenzymes in patients with an elevated serum alkaline phosphatase.

Collection Instructions

Container(s): Gold top (Serum Separator SST) 

Preferred Volume to Collect: 5 mL

Minimum Volume to Collect: 2 mL

Submission Container/Tube:  Plastic vial

Collection Instructions:

  • After collection, gently invert tube 8-10 times.
  • Specimen should be processed within 2 hours – See Processing Instructions below.



PROCESSING INSTRUCTIONS (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Serum

Centrifuge/Spin: Yes

Aliquot: Yes

Processing Instructions:

  • Allow blood to clot for 30 minutes in a vertical position and centrifuge promptly.
  • Within two hours aliquot serum into a plastic transport tube and refrigerate as soon as possible.
  • (+(2)S/PO/R)

Specimen Transport Temperature


Rejection Criteria

  • Plasma specimens. 
  • Grossly hemolyzed, lipemic or icteric specimens. 
  • Red-top tubes with serum not separated from cells within two hours of collection.
  • Specimens with total Alkaline Phosphatase levels lower than 111 U/L.

Specimen Stability

Serum Specimens (Pour-Overs)

Ambient: 2 hours

Refrigerated: 7 days

Test Frequency

3 times a week, afternoons.
Routine results available within 7 days.

Reference Range

Adult male & female:

Total Alkaline Phosphatase: 30-110 U/L.
Total Liver Fraction (Includes Liver 2): 25-74 U/L.
Liver 2 Fraction: 2-17 U/L.
Bone Fraction: 9-44 U/L.
Intestinal Fraction: 1-11 U/L.

Performing Department


Performing Department Laboratory Location

Beaumont Royal Oak Special Testing Laboratory


Separation by Agarose Gel Electrophoresis.


84075, 84080

CDM Code

3018407503, 3018408005

Epic Test ID




Reviewed Date