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Epic Code LAB1230916 Total Complement (CH50)

Additional Codes

Beaumont Code: LAB7024


Interface Order Alias


Clinical Information

Total Complement (CH50) is a functional assay that measures the activity of complement components C1 to C9 (i.e., the classical and terminal pathways). It does not measure the functional activity of the alternative pathway. If the Total Complement (CH50) measurement is zero or very low it is advisable to repeat the test to confirm the result. If the result is confirmed, the clinical presentation should be considered prior to further testing. If the patient exhibits recurrent neisserial infection, immunochemical determination of C5, C6, C7 and C8 is advised (or a functional assay for C5-C8). If the clinical problem is angioedema, initial studies should include C3, C4, C1 inhibitor (and possibly C1q and C2). Acquired disorders such as immune-mediated glomerulonephritis and other autoimmune disorders should direct testing towards C3 and C4.

Collection Instructions


Container(s): Red top (Serum gel/SST are not acceptable)

Preferred Volume to Collect: 6 mL

Minimum Volume to Collect: 2 mL

Submission Container/Tube: Plastic vial

Collection Instructions:

  • Specimen should be processed immediately (within 1 hour) – See Processing Instructions


PROCESSING INSTRUCTIONS (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Serum

Centrifuge/Spin: Yes

Aliquot: Yes

Processing Instructions:

  • Allow blood to clot for 30 minutes in a vertical position and centrifuge promptly.
  • Within an hour aliquot serum into a plastic transport tube and freeze as soon as possible.
  • (+(1)S/PO/F)

Specimen Transport Temperature


Rejection Criteria

  • Specimens collected in any tube other than a plain Red-top tube.
  • Specimens not collected and processed as indicated.
  • Specimens not received frozen from off-site areas/offices.
  • Severely hemolyzed, lipemic or icteric specimens.

Specimen Stability

Serum Specimens (Pour-Overs)
Frozen : 14 days

Test Frequency

Tuesday, Thursday.
Results available within 3 business days.

Reference Range

Greater than or equal to 41.7 U/mL.

Performing Department


Performing Department Laboratory Location

Beaumont Royal Oak Special Chemistry Laboratory


Liposome Immunoassay (The Binding Site SPAPLUS Analyzer)



CDM Code


Epic Test ID




Reviewed Date