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Epic Code LAB1230931 HLA Donor Typing Class I

Important Note

Corewell Health Reference Laboratory East Test

Test Name Alias

Donor HLA Class I Typing, HLA typing, tissue typing, HLA-A, B, C typing, DITYP

Interface Order Alias

1230931

Clinical Information

HLA Class I genotyping defines the antigens of the HLA-A, B and C loci of the Major Histocompatibility Complex found on the short arm of chromosome 6. Using DNA extracted from leukocytes, HLA-A, B, and C alleles are amplified via PCR using sequence-specific primers. After amplification, the specific HLA antigens are determined by gel electrophoresis. HLA genotyping serves an important role in transplantation and disease association studies.

Specimen Requirements

Specimen Collection Criteria

 

  • Collect: Two Lavender-top EDTA tubes OR one Pink-top tubes OR one Yellow-top ACD tubes.

 

  • Invert several times to mix well.

 

Physician Office/Draw Specimen Preparation

 

  • Do not refrigerate or freeze specimen. Maintain whole blood at room temperature (20-26°C or 68-78.8°F) prior to transport. Transport to the Laboratory within 72 hours of collection.

 

Preparation for Courier Transport

 

  • Transport: Whole blood at room temperature (20-25°C or 68-77°F).

 

Rejection Criteria

 

  • Unlabeled specimens.
  • Specimens drawn in clot, SST, or Sodium Heparin tubes.

 

In-Lab Processing

 

  • Maintain specimen at room temperature (20-26°C or 68-78.8°F) until testing.

Specimen Stability

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 72 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Frozen (-80°C): 2 years (DNA)

Test Frequency

As needed.
Results typically available within 5 business days.

Performing Department

REFERRAL SEND OUTS (West)

HLA (East)

Performing Department Laboratory Location

Royal Oak HLA Laboratory

Methodology

Polymerase Chain Reaction (PCR).

CPT

81372

CDM Code

HC HLA CLASS I TYPING LOW RESOLUTION COMPLETE [81372 (CPT®)]

Epic Test ID

1230102600

Reviewed Date

11/7/2024

Interpretation

By report.