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LAB COMMUNICATIONS 

 

COLLECTION & HANDLING

 

SPECIMEN TRANSPORTATION

Please call 616.774.7721 for specimen pick up

 

GENERAL INFORMATION

 

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Mayo Clinic Laboratories

Epic Code LAB1230931 HLA Donor Typing Class I

Important Note

Corewell Health Reference Laboratory East Test

Test Name Alias

Donor HLA Class I Typing, HLA typing, tissue typing, HLA-A, B, C typing, DITYP

Interface Order Alias

1230931

Clinical Information

HLA Class I genotyping defines the antigens of the HLA-A, B and C loci of the Major Histocompatibility Complex found on the short arm of chromosome 6. Using DNA extracted from leukocytes, HLA-A, B, and C alleles are amplified via PCR using sequence-specific primers. After amplification, the specific HLA antigens are determined by gel electrophoresis. HLA genotyping serves an important role in transplantation and disease association studies.

Specimen Requirements

Specimen Collection: Blood

 

Container(s): 6 mL Pink Top (EDTA) or TWO 3 mL Lavender Top (EDTA)

Preferred Volume to Collect: 6.0 mL

Collection Instructions:

  • Gently invert EDTA tube(s) 8 - 10 times after collection.

 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

  • Processed Specimen: Whole Blood
  • Centrifuge/Spin: No
  • Aliquot: No
  • Do not refrigerate or freeze specimen. Maintain whole blood at room temperature (20-26°C or 68-78.8°F) prior to transport. Transport to the Laboratory within 72 hours of collection.
  • Transport: Ambient

Specimen Stability

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 72 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Frozen (-80°C): 2 years (DNA)

Test Frequency

As needed.
Results typically available within 5 business days.

Reference Range

Interpretation by report.

Performing Department

Send Out to Corewell Health East

Performing Department Laboratory Location

Corewell Health East Royal Oak HLA Laboratory

Methodology

Polymerase Chain Reaction (PCR).

CPT

81372

CDM Code

HC HLA CLASS I TYPING LOW RESOLUTION COMPLETE [81372 (CPT®)]

Epic Test ID

1230102600

Reviewed Date

11/7/2024