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Epic Code LAB1230935 HLA Class II Typing

Important Note

Corewell Health Reference Laboratory East Test

Test Name Alias

DNA-based histocompatibility testing, disease association, histocompatibility, HLA DR testing, Donor HLA typing, Class II Typing, CIITY

Interface Order Alias

1230935

Clinical Information

HLA Class II genotyping defines the antigens of the HLA-DRB1 and DQB1 loci of the Major Histocompatibility Complex found on the short arm of chromosome 6. Using DNA extracted from leukocytes, HLA-DRB1 and DQB1 alleles are amplified via PCR using sequence-specific primers. After amplification, the specific HLA antigens are determined by gel electrophoresis. HLA genotyping serves an important role in transplantation and disease association studies.

 

Specimen Requirements

Specimen Collection Criteria

 

  • Collect: Two Lavender-top EDTA tubes OR one Pink-top OR one Yellow-top ACD tubes.

 

  • Invert several times to mix well.

 

Physician Office/Draw Specimen Preparation

 

  • Do not refrigerate or freeze specimen. Maintain whole blood at room temperature (20-26°C or 68-78.8°F) prior to transport. Transport to the Laboratory within 72 hours of collection.

 

Preparation for Courier Transport

 

  • Transport: Whole blood at room temperature (20-25°C or 68-77°F).

 

Rejection Criteria

 

  • Unlabeled specimens.
  • Specimens drawn in clot, SST, or Sodium Heparin tubes. 

 

In-Lab Processing

 

  • Maintain specimen at room temperature (20-26°C or 68-78.8°F) until testing.

Specimen Stability

Specimen Stability for Testing:

Room Temperature (20-26°C or 68-78.8°F): 72 hours
Refrigerated (2-8°C or 36-46°F): Unacceptable
Frozen (-20°C/-4°F or below): Unacceptable

Specimen Storage in Department Prior to Disposal:

Frozen (-80°C): 2 years (DNA)

Test Frequency

As needed.
Results typically available within 5 business days.

Performing Department

REFERRAL SEND OUTS (West)

HLA (East)

Performing Department Laboratory Location

Royal Oak HLA Laboratory

Methodology

Polymerase Chain Reaction (PCR).

CPT

81376

CDM Code

CMD

Epic Test ID

1230102604

Reviewed Date

11/7/2024

Interpretation

By report.