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HelpEpic Code LAB1231375 Alkaline Phosphatase Isoenzymes
Test Name Alias
Alkaline Phosphatase Fractionation | Fractionated Alkaline Phosphatase | Alk Phos
Interface Order Alias
1231375
Clinical Information
This assay is used to differentiate between liver, bone and intestinal isoenzymes in patients with an elevated serum alkaline phosphatase.
Collection Instructions
Container(s): Gold top (Serum Separator SST)
Preferred Volume to Collect: 5 mL
Minimum Volume to Collect: 2 mL
Submission Container/Tube: Plastic vial
Collection Instructions:
- After collection, gently invert tube 8-10 times.
- Specimen should be processed within 2 hours – See Processing Instructions below.
PROCESSING INSTRUCTIONS (Laboratory, Outpatient or Off-site collection)
Processed Specimen: Serum
Centrifuge/Spin: Yes
Aliquot: Yes
Processing Instructions:
- Allow blood to clot for 30 minutes in a vertical position and centrifuge promptly.
- Within two hours aliquot serum into a plastic transport tube and refrigerate as soon as possible.
- (+(2)S/PO/R)
Specimen Transport Temperature
Refrigerated
Rejection Criteria
- Plasma specimens.
- Grossly hemolyzed, lipemic or icteric specimens.
- Red-top tubes with serum not separated from cells within two hours of collection.
- Specimens with total Alkaline Phosphatase levels lower than 111 U/L.
Specimen Stability
Serum Specimens (Pour-Overs)
Ambient: 2 hours
Refrigerated: 7 days
Test Frequency
3 times a week, afternoons.
Routine results available within 7 days.
Reference Range
Adult male & female:
Total Alkaline Phosphatase: 30-110 U/L.
Total Liver Fraction (Includes Liver 2): 25-74 U/L.
Liver 2 Fraction: 2-17 U/L.
Bone Fraction: 9-44 U/L.
Intestinal Fraction: 1-11 U/L.
Performing Department
REFERRAL SEND OUTS
Performing Department Laboratory Location
Beaumont Royal Oak Special Testing Laboratory
Methodology
Separation by Agarose Gel Electrophoresis.
CPT
84075, 84080
CDM Code
3018407503, 3018408005
Epic Test ID
123010748
LOINC
6768-6
Reviewed Date
6/9/2023