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LAB COMMUNICATIONS 

 

COLLECTION & HANDLING

 

SPECIMEN TRANSPORTATION

Please call 616.774.7721 for specimen pick up

 

GENERAL INFORMATION

 

GENERAL TEST INFORMATION

 

BILLING

 

WEBSITES

Mayo Clinic Laboratories

Epic Code LAB1231583 Vitamin D 25-Hydroxy (25-OH Vitamin D)

Test Name Alias

Vitamin D 25-Hydroxy Blood Level, Vitamin D25LvL, Vitamin D25 Blood Level, Vitamin D

Interface Order Alias

1231583

Clinical Information

  • Vitamin D (25-Hydroxy) is the preferred test to diagnose Vitamin D insufficiency and monitor response to therapy.
  • Testing is recommended only for patients at risk for Vitamin D insufficiency.

Collection Instructions

Specimen Collection: Blood

 

Container(s): Gold top (Serum Separator SST), Red top acceptable

Preferred Volume to Collect: 5.0 mL

Minimum Volume to Collect: 1.0 mL

Neonate Volume to Collect: 0.5 mL

Capillary collect ok? Yes

Microtainer acceptable: Yes

 

Collection Instructions:

  • After collection, gently invert tube 8-10 times.
  • Specimen should be processed within 2 hours – See Processing Instructions below.

 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

  • Processed Specimen: Serum
    • Allow blood to clot for 30 minutes and centrifuge within 2 hours.
  • Centrifuge/Spin: Yes
  • Aliquot: No
  • Minimum serum volume: 0.5 mL (neonates may submit less)
  • Transport Temperature: Refrigerated

Rejection Criteria

  • Not centrifuged promptly after collection.
  • Quantity not sufficient.

Specimen Stability

Ambient: 8 hours

Refrigerate: 3 days

Frozen: Freeze for longer storage

Laboratory Retention: 3 days

Test Frequency

Available 24 hours, usual TAT 1 day.

Reference Range

≤20 ng/mL - Deficiency 
21 - 29 ng/mL - Insufficiency  
30 - 100 ng/mL - Optimal Level 
>100 ng/mL - Possible Toxicity 

Performing Department

Chemistry

Performing Department Laboratory Location

Corewell Health Reference Laboratory, Grand Rapids

Methodology

Electrochemiluminescence Immunoassay (ECLIA)

CPT

82306

CDM Code

    3018230601

Epic Test ID

123010951

LOINC

62292-8

Reviewed Date

5/30/2025

Updated Date

6/5/2025 - codes added