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Epic Code LAB2111389 FKHR (FOX01) (13q14) Rearrangement by FISH

Test Name Alias

FKHR (13q14) Rearrangement by FISH | FKHR Break Apart | FOX01A (13q14) by FISH | 952

Interface Order Alias

11403

Quick Collect

AP Ambient

Clinical Information

Fluorescence in situ hybridization (FISH) performed using FKHR (13q14) Dual Color Rearrangement Probe. This probe is useful for detection of rearrangements of the FKHR (FOXO1) gene region associated with alveolar rhabdomyosarcomas.

Routine chromosome analysis is performed on all fresh tumor specimens to exclude abnormalities not identified by the specified FISH probe(s).

Collection Instructions

Multiple specimen collection types. Submit only one:

 

Specimen Collection: Formalin-fixed, paraffin-embedded (FFPE) tissue

Container(s): Block

Preferred Volume to Collect: 4 unstained slides cut between 4 and 6 microns thick

Collection Instructions:

  • Include an H&E slide scribed by Pathologist and Surgical Specimen Testing order sheet.
  • Place specimen in 10% Formalin and order Tissue Pathology in addition to FKHR (FOX01) (13q14) Rearrangement by FISH order.

 

Specimen Collection: Fine Needle Aspirate (FNA, such as lymph node)

Collection Instructions: Order FNA Cytology (refer to test code 202 for collection instructions) in addition to FKHR (FOX01) (13q14) Rearrangement by FISH order.

 

 

Specimen Collection:  Pleural Fluid

Collection instructions:  Place specimen in sterile container and order Medical Cytology in addition to FKHR (FOX01) (13q14) Rearrangement by FISH order.

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Spin: N

Aliquot: N

 

Transport Temperature: Ambient

 

If ambient temperature exceeds 80°F or is below 40°F, please send in an insulated container. All samples should be directed to the laboratory as soon as possible.

Specimen Stability

Ambient: 3 days for fresh specimens (beyond 3 days, specimen viability will be detemined by Cytogenetics Lab)

Laboratory Retention: primary specimens 4 weeks; frozen pellets retained greater than 1 year

Test Frequency

Test Set Up: Monday – Friday 8:00 am – 5:00 pm, Saturday 8:00 am – 3:00 pm.

Technologist on call for Sunday set up.

Final report: 2 – 5 days.

Reference Range

An interpretative report will be provided.

Performing Department

Cytogenetics

Performing Department Laboratory Location

Corewell Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Performed by fluorescence in situ hybridization (FISH). This test was developed and its performance characteristics determined by Spectrum Health Cytogenetics Laboratory. It has not been cleared or approved by the US FDA. The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes.

CPT

88275, 88271 x2

CDM Code

3108827102, 3108827501

Epic Test ID

1230100693

LOINC

Specimen Type: 66746-9

Indication Provided: 42349-1

Result: in process

Interpretation: 59050-5

Comments: 69965-2

Reviewed Date

4/28/2023