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Epic Code LAB2111406 MLL (11q23) Rearrangement by FISH

Test Name Alias

MLL (11q23) Rearrangement by FISH | MLL Break Apart | 966

Interface Order Alias

11420

Quick Collect

AP Ambient

Clinical Information

Fluorescence in situ hybridization (FISH) using the MLL DNA probe can detect rearrangements of 11q23 frequently associated with myeloid and lymphoid leukemia. FISH can be done on metaphase cells.

 

Routine chromosome analysis is performed on all diagnostic samples and is recommended for all bone marrow specimens to exclude abnormalities not identified by the specified FISH probe(s).

Collection Instructions

Multiple specimen collection types. Submit only one.

 

Specimen Collection: Bone Marrow

Container(s): Green Top (Sodium Heparin)

Preferred volume to collect: 5.0 mL

Minimum volume to collect: 1.0 mL

Neonate volume to collect: 1.0 mL

Collection Instructions:

  • Collect bone marrow and transfer marrow from syringe to plain green top sodium heparin tube. 

 

Specimen Collection: Peripheral blood
Container/Tube: Green Top (Sodium Heparin)

Preferred volume to collect: 8.0 mL

Minimum volume to collect: 0.5 mL

Neonate volume to collect: 0.5 mL

Collection Instructions:

  • Peripheral blood may be substituted for Bone Marrow if circulating blasts are present 
  • Collect peripheral blood into a plain green top sodium heparin tube. 
  • Keep sample at room temperature and send to the Lab immediately. 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Spin: N

Aliquot: N

 

Transport Temperature: Ambient

 

If ambient temperature exceeds 80°F or is below 40°F, please send in an insulated container. All samples should be directed to the laboratory as soon as possible.

Specimen Stability

Ambient: 3 days (beyond 3 days, specimen viability will be detemined by Cytogenetics Lab)

Laboratory Retention: primary specimens 4 weeks; frozen pellets retained greater than 1 year

Test Frequency

Test Set Up: Monday – Friday 8:00 am – 5:00 pm, Saturday 8:00 am – 3:00 pm.

Technologist on call for Sunday set up.

Final report: 2 – 3 days.

Reference Range

An interpretive report will be provided.

Performing Department

Cytogenetics

Performing Department Laboratory Location

Corewell Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Performed by fluorescence in situ hybridization (FISH). This test was developed and its performance characteristics determined by Spectrum Health Cytogenetics Laboratory. It has not been cleared or approved by the US FDA. The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes.

CPT

88275, 88271 x2

CDM Code

3108827102, 3108827501

Epic Test ID

1230100950

LOINC

Specimen Type: 66746-9

Indication Provided: 42349-1

Result: in process

Interpretation: 59050-5

Comments: 69965-2

Reviewed Date

4/28/2023