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Epic Code LAB303 Factor II Activity

Important Note

For PT G20210A/Factor II Mutation please order Prothrombin G20210A, test #LAB8340

There is currently a nationwide shortage of this tube. Please take steps to ensure specimen integrity to reduce recollection. Specimen Tube Shortage (link)

Test Name Alias

Factor II (2) Activity | 4273 | Factor 2

Interface Order Alias

10373

Quick Collect

+(1)S10POx3/F

Clinical Information

Factor II activity can be used for the following:  to evaluate congenital or acquired deficiencies, monitor therapy of prothrombin concentrate, monitor oral anticoagulants, and the evaluation of protein synthesis in liver disease.

Collection Instructions

Specimen Collection: Blood

 

Container(s): Blue top (3.2% Na. Citrate)

  • Preferred Volume to Collect: 2.7 mL (full tube)
  • Minimum Volume to Collect: 1.8 mL (full tube)
  • Capillary collect ok: No
  • Microtainer acceptable: No

 

COLLECTION INSTRUCTIONS

  • Detailed instructions (link): Anticoagulant Correction for High Hematocrit

  • Detailed instructions (link): Blue Citrate Tube

  • Tube must be full. Do not over fill or under fill; look for the fill indicator lines.

  • Gently invert 8 - 10 times to mix anticoagulant with blood.
  • Specimen should be processed within 1 hour – See Processing Instructions below.

 

PROCESSING INSTRUCTIONS (Laboratory, Outpatient or Off-site collection)

 

Processed Specimen: Platelet Poor Plasma with a platelet count <10 X 109/L

Centrifuge/Spin: Yes

Aliquot: Yes

Processing Instructions:

  • Centrifuge and remove plasma within 1 hour of collection.
  • Separate plasma into 2-3 aliquots, minimum of 0.5 mL each.
  • Freeze aliquots in plastic transport tubes immediately.

Transport Temperature:

  • Ambient (up to 1 hour in whole blood state)
  • Frozen (plasma only >4 hours)

Rejection Criteria

  • Hemolysis
  • Specimen has been thawed

Specimen Stability

Ambient: 1 hour

Frozen: 2 months

Laboratory Retention: 2 months

Test Frequency

Available Monday – Friday, usual TAT 1-4 days.

Reference Range

Adult: 50 – 150 %

 

Pediatric reference ranges have not been established.

 

Critical Values Table

Performing Department

Coagulation

Performing Department Laboratory Location

Corewell Health Reference Laboratory, Grand Rapids, MI

Methodology

Prothrombin (PT) clot based assay with plasma deficient substrate

CPT

85210

CDM Code

3058521001

Epic Test ID

1230100667

LOINC

Factor II Activity: 3289-6

Mayo Access Code

SHO10373

Reviewed Date

3/23/2023

Updated Date

9/5/24 - processing instructions