Sign in →

Epic Code LAB304 Factor V Activity

Important Note

Please verify with the ordering physician for all orders listed as "Factor V". 

Factor V activity is useful for diagnosing congenital or acquired factor V deficiencies.  Factor V activity is not indicated for hyper coagulation evaluation.   For hypercoagulation panels please order Factor V Leiden DNA analysis. 

There is currently a nationwide shortage of this tube. Please take steps to ensure specimen integrity to reduce recollection. Specimen Tube Shortage (link)

Test Name Alias

Coag Factor V (5) Activity | 4116 | Factor 5

Interface Order Alias

10375

Quick Collect

+(1)S10/POx3/F

Clinical Information

The evaluation of coagulation factor V is indicated for the diangosis of congenital or acquired Factor V deficient states.  Acquired deficiencies are noted in cases of liver dysfunction, consumption coagulopathy, factor V inhibitors, and myeloproliferative disorders. Also indicated for the evaluation of a prolonged prothrombin time or activated partial thromboplastin time.

Ordering Instructions

For written orders: Please specify Factor V Activity or Factor V Leiden DNA Analysis.

Factor V activity is useful for diagnosing congenital or acquired factor V deficiencies.  Factor V activity is not indicated for hyper coagulation evaluation.   For hypercoagulation panels please order Factor V Leiden DNA analysis. 

Collection Instructions

Specimen Collection: Blood

 

Container(s): Blue top (3.2% Na. Citrate)

Preferred Volume to Collect: 2.7 mL (full tube)

Minimum Volume to Collect: 1.8 mL (full tube)

Capillary collect ok: No

Microtainer acceptable: No

 

Collection Instructions:

  • Detailed instructions (link): Anticoagulant Correction for High Hematocrit

  • Detailed instructions (link): Blue Citrate Tube

  • Tube must be full. Do not over fill or under fill; look for the fill indicator lines.

  • Gently invert 8 - 10 times to mix anticoagulant with blood.
  • Specimen should be processed within 1 hour – See Processing Instructions below.
  • +(1)S10/POx3/F

 

 

PROCESSING INSTRUCTIONS (Laboratory, Outpatient or Off-site collection)

 

Processed Specimen: Platelet Poor Plasma with a platelet count <10 X 109/L

Centrifuge/Spin: Yes

Aliquot: Yes

Processing Instructions:

  • Centrifuge and remove plasma within 1 hour of collection.
  • Separate plasma into 2-3 aliquots, minimum of 0.5 mL each.
  • Freeze aliquots in plastic transport tubes immediately.

Transport Temperature:

  • Ambient (up to 1 hour in whole blood state)
  • Frozen (plasma only >4 hours)

Rejection Criteria

  • Hemolysis
  • Specimen has been thawed

Specimen Stability

Ambient: 1 hour

Frozen: 2 months

Laboratory Retention: 2 months

Test Frequency

Available Monday-Friday, usual TAT 1-4 days.

Reference Range

Adult: 50 – 150 %

 

Pediatric reference ranges have not been established.

 

Critical Values Table

Performing Department

Coagulation

Performing Department Laboratory Location

Corewell Health Reference Laboratory, Grand Rapids, MI

Methodology

Prothrombin (PT) clot based assay with plasma deficient substrate

CPT

85220

CDM Code

3058522001

Epic Test ID

1230100505

LOINC

Factor V Activity: 3193-0

Mayo Access Code

SHO10375

Reviewed Date

3/23/2023

Updated date

9/5/24 - Processing Instructions