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Epic Code LAB308 Factor IX Activity

Important Note

Please take steps to ensure specimen integrity to reduce recollection. Specimen Tube Shortage (link)

Test Name Alias

Factor IX (9) Activity | Hemophilia B | 4115 | Factor 9

Interface Order Alias

10374

Quick Collect

+(1)S10POx3/F

Clinical Information

Factor IX activity can be used to clarify the cause of a prolonged partial thromboplastin time.  It is useful for diagnosing inherited or acquired factor deficiencies.  It may be used to monitor substitution therapy for Hemophilia B, the evaluation of consumption coagulopathy, hepatic cirrhosis, and monitoring of oral anticoagulant. 

Collection Instructions

Specimen Collection: Blood

 

Container(s): Blue top (3.2% Na. Citrate)

  • Preferred Volume to Collect: 2.7 mL (full tube)
  • Minimum Volume to Collect: 1.8 mL (full tube)
  • Capillary collect ok: No
  • Microtainer acceptable: No

 

COLLECTION INSTRUCTIONS

  • Detailed instructions (link): Anticoagulant Correction for High Hematocrit
  • Detailed instructions (link): Blue Citrate Tube
  • Tube must be full. Do not over fill or under fill; look for the fill indicator lines.
  • Gently invert 8 - 10 times to mix anticoagulant with blood.
  • Specimen should be processed within 1 hour – See Processing Instructions below.

 

PROCESSING INSTRUCTIONS (Laboratory, Outpatient or Off-site collection)

 

Processed Specimen: Platelet Poor Plasma with a platelet count <10 X 109/L

Centrifuge/Spin: Yes

Aliquot: Yes

Processing Instructions:

  • Centrifuge and remove plasma within 1 hour of collection.
  • Separate plasma into 3 aliquots, minimum of 0.5 mL each.
  • Freeze aliquots in plastic transport tubes immediately.

Transport Temperature:

  • Ambient (up to 1 hour in whole blood state)
  • Frozen (plasma only >4 hours)

Rejection Criteria

  • Hemolysis
  • Specimen has been thawed

Specimen Stability

Ambient: 1 hour

Frozen: 2 months

Laboratory Retention: 2 months

Test Frequency

Available Monday – Friday, usual TAT 1-4 days.

Reference Range

Adult: 50 – 150 %

 

Pediatric reference ranges have not been established.

 

Critical Values Table

Performing Department

Coagulation

Performing Department Laboratory Location

Corewell Health Reference Laboratory, Grand Rapids, MI

Methodology

Activated Partial Thromboplastin (APTT) clot based assay with plasma deficient substrate

CPT

85250

CDM Code

3058525001

Epic Test ID

1230100668

LOINC

Factor IX Activity: 3187-2

Mayo Access Code

SHO4115

Reviewed Date

3/23/2023

Updated Date

9/7/2024 - 262 - Processing Instructions