Epic Code LAB326 Mixing Studies, APTT
Test Name Alias
Mixing Studies APTT | 4120
Interface Order Alias
10524
Quick Collect
+(1)S10/POx3/F
Clinical Information
The mixing study is used to evaluate a prolonged activated partial thromboplastin time. It is an effective screening tool for coagulation factor deficiencies or abnormalities in the intrinsic and common pathway. Additionally, it is utilized to detect coagulation inhibitors.
Icons & Photos
3.2% Na Citrate Tube
Collection Instructions
Specimen Collection: Blood
Container(s): (3) 3.2% Na Citrate Tube (blue top)
Preferred Volume to Collect: 8.1 mL (3 full 2.7 mL tubes)
Minimum Volume to Collect: 5.4 mL (3 full 1.8 ml tubes)
Capillary collect ok: No
Microtainer acceptable: No
Collection Instructions:
-
Detailed instructions (link): Blue Citrate Tube
- Specimen should be processed within 1 hour – See Processing Instructions below.
PROCESSING INSTRUCTIONS (Laboratory, Outpatient or Off-site collection)
Processed Specimen: Platelet Poor Plasma with a platelet count <10 X 109/L
Centrifuge/Spin: Yes
Aliquot: Yes
Processing Instructions:
- Centrifuge and remove plasma within 1 hour of collection.
-
For detailed instructions to prepare platelet poor plasma please use the following (link): Preparing Platelet Poor Plasma
-
Detailed instructions (link): Anticoagulant Correction for High Hematocrit
-
- Separate plasma into 4-6 aliquots, minimum of 0.5 mL each.
- Freeze aliquots in plastic transport tubes immediately.
Transport Temperature:
- Ambient (up to 1 hour in whole blood state)
- Frozen (plasma only >1 hour)
Rejection Criteria
- Hemolysis
- Specimen has been thawed
Specimen Stability
- Ambient: 1 Hour Whole Blood
- Frozen: 2 months – Platelet poor plasma; aliquoted and frozen within 1 hour of collection
- Laboratory Retention: 2 months
Reflex Information
Link: Mandatory Reflex Testing approved by MEC
Reflex testing may include the following: lupus screen and associated tests.
Test Frequency
Available Monday-Friday, usual TAT 1-4 days.
Reference Range
-
24.0 - 34.0 seconds (Corewell Health Reference Lab, Grand Rapids, CS 5100 Analyzer)
-
22.0 - 27.0 seconds (Corewell Health Reference Lab, Grand Rapids, BCS XP analyzer)
Performing Department
Coagulation
Performing Department Laboratory Location
Corewell Health Reference Laboratory, Grand Rapids, MI
Methodology
Activated partial thromboplastin clot based assay.
CPT
85730
CDM Code
3058573001
Epic Test ID
1230100945
Mayo Access Code
SHO4120
Reviewed Date
3/23/2023
Updated Date
09/26/2023, 09:08: Reference Range
9/5/2024: Processing Instructions