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Epic Code LAB3417 Group B Streptococcus (GBS), PCR

Important Note

This test is only intended for women of child bearing age.

Test Name Alias

GBS PCR | 4003

Interface Order Alias


Icons & Photos

Standard Tip ESwab 

Collection Instructions

Specimen Type: Vaginal/Rectal

Container/Tube: ESwab

Collection Instructions: Indicate source of specimen. Specimen is unacceptable if received without fluid or swab in the transport tube.

  1. Open the peel pouch and remove the contents. Aseptically unscrew and remove the cap from the ESwab.

  2. Place the tube in a secure position so as not to allow the liquid to leak out of the tube.

  3. Remove the swab. Collect the patient's sample using the swab. (If vaginal/rectal swab, collect a specimen from the vaginal area and then collect from the rectal area.)

  4. Insert the swab back into the tube of liquid, all the way to the bottom of the tube.

  5. Holding the swab shaft close to the rim of the tube, break the applicator shaft at the color breakpoint indication line.

  6. Screw the cap on tightly to prevent leakage. Discard the broken handle of the shaft into an approved medical waste disposal container.

  7. Write the patient's information (Name and Date of Birth) on the tube or apply a label with patient’s information. Indicate source.

  8. Send specimen to the Laboratory.


Specimen Transport Temperature: Ambient


NOTE: The ESwab fluid maintains the viability of diverse bacteria. Do not send a dry ESwab as this will lead to unsatisfactory results. If the tube spills its contents prior to inserting the swab, the liquid is non-toxic. Simply put the swab into another tube before sending it to the laboratory and discard the spilled tube.

Rejection Criteria

Cervical, Perianal, Perirectal or Perineal specimens will be rejected

Specimen Stability

To maintain optimum organism viability, transport specimens directly to the lab preferably within 2 hours of collection

Ambient: up to 48 hours

Refrigerated: up to 48 hours

Frozen: Do Not freeze

Laboratory Retention: 4 days

Test Frequency

Available daily, usual TAT 24 - 48 hours after receipt of specimen in Microbiology Department.

Reference Range

Negative (No Group B Streptococcus detected)

Positive (Group B Streptococcus detected)

Performing Department


Performing Department Laboratory Location

Corewell Health Reference Laboratory, Grand Rapids, MI


Polymerase chain reaction (PCR)

Vag/rectal swabs are inoculated into the LIM broth and are incubated for 18 to 24 hours prior to performing the PCR.



CDM Code


Epic Test ID




Mayo Access Code


Additional Information

This test applies to specimens sent to

SHRL (Spectrum Health Regional Laboratory, Main Laboratory, Medical Center, 35 Michigan, Grand Rapids)

BIRL (Blodgett Immediate Response Laboratory, Grand Rapids)

SHRCH (Spectrum Health Reed City Hospital)

SHZCH (Spectrum Health Zeeland Community Hospital)


Reference Material:

BBL™ Lim Broth PI, January 2011

BD ESwab Collection, January 2010



Reviewed Date