Epic Code LAB3417 Group B Streptococcus (GBS), PCR
Test Name Alias
GBS PCR | 4003
Interface Order Alias
11134
Icons & Photos
Standard Tip ESwab
Collection Instructions
Specimen Type: Vaginal/Rectal
Container/Tube: ESwab
Collection Instructions: Indicate source of specimen. Specimen is unacceptable if received without fluid or swab in the transport tube.
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Open the peel pouch and remove the contents. Aseptically unscrew and remove the cap from the ESwab.
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Place the tube in a secure position so as not to allow the liquid to leak out of the tube.
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Remove the swab. Collect the patient's sample using the swab. (If vaginal/rectal swab, collect a specimen from the vaginal area and then collect from the rectal area.)
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Insert the swab back into the tube of liquid, all the way to the bottom of the tube.
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Holding the swab shaft close to the rim of the tube, break the applicator shaft at the color breakpoint indication line.
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Screw the cap on tightly to prevent leakage. Discard the broken handle of the shaft into an approved medical waste disposal container.
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Write the patient's information (Name and Date of Birth) on the tube or apply a label with patient’s information. Indicate source.
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Send specimen to the Laboratory.
Specimen Transport Temperature: Ambient
NOTE: The ESwab fluid maintains the viability of diverse bacteria. Do not send a dry ESwab as this will lead to unsatisfactory results. If the tube spills its contents prior to inserting the swab, the liquid is non-toxic. Simply put the swab into another tube before sending it to the laboratory and discard the spilled tube.
Rejection Criteria
Cervical, Perianal, Perirectal or Perineal specimens will be rejected
Specimen Stability
Corewell Health Reference Laboratory, Grand Rapids, MI
To maintain optimum organism viability, transport specimens directly to the lab preferably within 2 hours of collection
Ambient: up to 48 hours
Refrigerated: up to 48 hours
Frozen: Do Not freeze
Laboratory Retention: 4 days
Corewell Health Lakeland St. Joseph Laboratory, St. Joseph, MI
Ambient: eswabs may be stored for up to 24 hours before processing in Lim broth.
Refrigerate:
• eswabs may be stored for up to two days before processing in Lim broth.
• Enriched Lim broth is stable for up to 72 hours.
Laboratory Retention: 7 days
Test Frequency
Available daily, usual TAT 24 - 48 hours after receipt of specimen in Microbiology Department.
Reference Range
Negative (No Group B Streptococcus detected)
Positive (Group B Streptococcus detected)
Performing Department
Microbiology
Performing Department Laboratory Location
Corewell Health Reference Laboratory, Grand Rapids, MI
Corewell Health Lakeland St. Joseph Laboratory, St. Joseph, MI
Methodology
Polymerase chain reaction (PCR)
Vag/rectal swabs are inoculated into the LIM broth and are incubated for 18 to 24 hours prior to performing the PCR.
CPT
87653
CDM Code
3068765301
Epic Test ID
1230102159
LOINC
LOINC
Mayo Access Code
SHO11134
Additional Information
Reference Material:
BBL™ Lim Broth PI, January 2011
BD ESwab Collection, January 2010
Luminex-Aries, www.luminexcorp.com
Remel ™ Todd Hewitt Broth w/ Colistin and Nalidixic Acid (LIM Broth) PI, Revised July 2, 2008
• Cepheid ®, www.cepheid.com
Reviewed Date
9/7/2023
Updated Date
4/10/2025 - CHS info added