Epic Code LAB348 Urinalysis (UA) with Reflex to Culture if Positive (Outpatient Only)
Test Name Alias
Urinalysis, Culture if Indicated | UA | Urine C&S | UA do if | Urinalysis w/Micro Exam, C&S if Indicated | 115
Interface Order Alias
10540
Quick Collect
CC/Random+(1)R
Clinical Information
Refrigeration of urine inhibits bacteria growth but does not prevent the lytic effects of low specific gravity or alkaline pH. Urine crystal formation may be induced by refrigeration.
Ordering Instructions
Please do not order UA do if and Urine culture together. UA do if will reflex to Urine Culture if necessary. Please indicate source, i.e. ccms, nephrostomy, straight catheter.
This test is only available for outpatient ordering.
Effective 11/17/2021: When an order for Urine Culture [LAB239] and Urinalysis, do Culture if indicated [LAB348] is placed in the same encounter, these labs will update at the time of collection to an order for Urine Culture [LAB239] and Urinalysis [LAB2111180] to decrease duplicate urine cultures.
Collection Instructions
Specimen Collection: Urine (Clean Catch Mid-Stream/CCMS or Straight Catheter)
Container(s): 2 tubes total
- 1 Pale Yellow Top Urine Tube (UA No Additive) AND
- 1 Gray top urine C&S tube (contains boric acid)
Acceptable containers: Sterile Cup AND 1 Gray top Urine C&S tube (contains boric acid), collected together.
Preferred Volume to Collect: 14 mL (1 x 10 mL Yellow and 1 × 4 mL Gray)
Minimum Volume to Collect: 4.0 mL
Neonate Volume to Collect: 3.0 mL
Collection Instructions:
- No preservatives
- Clean Catch Mid-Stream or Straight Catheter Collection.
- Ambient specimen must be received in the Lab within 2 hours.
- If delay: Keep the specimen refrigerated and send to laboratory as soon as possible.
- Specimen must be received in laboratory for analysis within 24 hours of collection.
- Gray vacutainer is to be used for Culture and Sensitivity (C&S).
- Detailed Instructions (link): Patient Collection Instructions
- Detailed Instructions (link): Urine Collection Guide for Staff
Unacceptable
Specimens with 10 or more squamous epithelial cells are not appropriate for culture, a CCMS or catheterized may be indicated if culture is requested.
Processing Instructions (Laboratory, Outpatient or Off-site collection)
- If available, two barcode labels will print at the time of collection. Apply one label to the container and place the second label in the pouch of the specimen bag.
- Transport Temperature:
- Ambient: Gray top tube, Yellow Tube
- Refrigerate: Sterile cup
Rejection Criteria
Specimens that do not meet Spectrum Health Laboratory standards may be rejected (cancelled) due to:
- Request a repeat specimen when there is no evidence of refrigeration for a sterile cup collection and the specimen is greater than 2 hours old.
- Foley catheter tips/bags
- Catheter bags
- Leaky containers
- Non-sterile container (example: pill bottles or household containers)
Specimen Stability
2 Yellow top tubes and Urine Cup:
Ambient: 2 hours
Refrigerate: 24 hours
Laboratory Retention: 3 days
Gray top tube:
Ambient: 48 hours
Refrigerate: 48 hours
Laboratory Retention: 3 days
Reflex Information
- Culture and Sensitivity (C&S) will be performed with 2 or more of the following abnormal findings, provided there are less than 10 squamous epithelial cells per HPF:
- Greater than or equal to 10 WBC
- Positive leukocyte esterase
- Positive nitrite
OR if the specimen is:
- Grossly bloody
- Specimens with 10 or more squamous epithelial cells, culture will not be performed
- Culture and Sensitivity (C&S) will be performed if volume is inadequate for microscopic exam and Urinalysis (UA) with one or more of the following abnormal findings:
- Positive leukocyte esterase
- Positive nitrate
- If urinalysis is negative, culture will not be performed.
Test Frequency
Available 24 hours, usual TAT 1 day
Reference Range
Urinalysis (UA) is always performed first
Reference Values Inpatient/Outpatient Adult/Pediatric
|
|
Test Name/Units of measure |
Normal Reference Range |
Appearance (Clarity) |
Clear |
Specific Gravity |
1.002 – 1.030 |
Urine pH |
5.0 – 9.0 |
Glucose (mg/dL) |
Negative, Normal |
Ketones (mg/dL) |
Negative |
Protein (mg/dL) |
Negative |
Blood |
Negative |
Bilirubin |
Negative, Ictotest negative |
Urobilinogen (mg/dL) |
0.2 – 1.0, Normal |
Nitrite (reductase) |
Negative |
Leukocyte Esterase |
Negative |
WBC (HPF) |
0-5/HPF |
WBC Clumps (HPF) |
Negative, Not Present |
RBC (HPF) |
0-3/HPF |
Bacteria (HPF) |
Negative, None |
Yeast (HPF) |
Negative, None, Not Present |
Mucus (LPF) |
Negative, None, Not Present |
Squamous Epithelial (HPF) |
0-9/HPF |
Renal Epithelial (HPF) |
Negative, None |
Transitional Epithelial (HPF) |
Negative, None |
Hyaline Casts (LPF) |
0-3/LPF |
Casts (LPF) |
Negative, None |
Amorphous Crystals (HPF) |
None to Heavy |
Crystals (HPF) |
Negative, None |
Sperm (HPF) |
Negative, None, Not Present |
Trichomonas (HPF) |
Negative, None |
Urine Fat (HPF) |
Negative, None |
Laboratory Critical Values Table
If reflexed to Urine Culture:
Negative culture: No bacteria isolated
Positive culture: Identification with susceptibility on urinary pathogens with colony counts of greater than 10,000 CFU/mL
Performing Department
Urines
Performing Department Laboratory Location
Corewell Health Reference Laboratory, Grand Rapids, MI
Corewell Health Big Rapids Laboratory, Big Rapids, MI
Corewell Health Blodgett Laboratory, Grand Rapids, MI
Corewell Health Gerber Laboratory, Fremont, MI
Corewell Health Kelsey Laboratory, Lakeview, MI
Corewell Health Ludington Laboratory, Ludington, MI
Corewell Health Pennock Laboratory, Hastings, MI
Corewell Health Reed City Laboratory, Reed City, MI
Corewell Health Greenville Laboratory, Greenville, MI
Corewell Health Zeeland Laboratory, Zeeland, MI
Corewell Health Lakeland St. Joseph Laboratory, St. Joseph, MI
Corewell Health Lakeland Niles Laboratory, Niles, MI
Corewell Health Lakeland Watervliet Laboratory, Watervliet, MI
Methodology
Methodology is available on request.
Please call the Laboratory Customer Service Call Center 616.774.7721 or send an email to LaboratoryServices@spectrumhealth.org
CPT
81001
This test is not limited to the CPT code(s) listed. Others may be added if culture and sensitivity is performed and if more testing is necessary depending on the pathogens isolated (such as MIC’s and ID’s).
CDM Code
3078100101
Epic Test ID
1230101265
Reviewed Date
9/5/2023