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Mayo Clinic Laboratories

Epic Code LAB3620 HIV-1 Quantitative RNA, PCR

Test Name Alias

human immunodeficiency virus | HIV Quant | HIV 1 RNA | HIV-1 RNA | HIV1 | HIV-1 Viral Load | 7125

Interface Order Alias

55082

Quick Collect

+(6)S/PO/F

Collection Instructions

Specimen Collection: Blood

 

Container(s): 6 mL Pink Top (EDTA) 

Preferred Volume to Collect: Full tube (6 mL)

Capillary collect ok? No

 

Collection Instructions:

  • Gently invert EDTA tube(s) 8 - 10 times after collection.
  • Specimen should be processed within 24 hours – See Processing Instructions.

 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

  • Processed Specimen: Plasma 
  • Centrifuge/Spin: Yes
  • Aliquot: Yes
  • Processes Minimum Volume: 1.6 mL Plasma 
  • Processing Instructions:
    • With room temperature storage, centrifuge whole blood in EDTA tube within 24 hours of collection. 
    • With refrigerated temperature storage, centrifuge whole blood in EDTA tube within 48 hours of collection. 
    • Transfer plasma to a screw-capped transport tube.
    • Plasma needs to be stored frozen in screw-capped transport tube after separation from cells
  • Transport temperature: Prefer that plasma be frozen for transport

Specimen Stability

Ambient: 24 hours

Refrigerated: Whole blood in EDTA is stable for 48 hours; plasma is stable for 72 hours. 

Frozen: 60 days

Laboratory Retention: 1 month

Test Frequency

Available once per week, usual TAT less than 7 days

Reference Range

Not detected: <20 copies/mL

 

Linear range of positive results

Positive: 20 to 10,000,000 (1e7) copies/mL

Equivocal: detected but below lower limit of quantitation (<20 copies/mL)

 

Performing Department

Molecular Diagnostics

Performing Department Laboratory Location

Corewell Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Quantitative Reverse Transcriptase Real-Time Polymerase Chain Reaction (RT-PCR)

CPT

87536

CDM Code

3008753601

Epic Test ID

1230100786

LOINC

Unavailable

 

Mayo Access Code

SHO55082

Reviewed Date

6/22/2023