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Epic Code LAB3640 JAK2 V617F Mutation, do MPN if Negative

Important Note

The same sample may be used for both JAK2 V617F and MPN Expanded Panel testing.

Test Name Alias

MPN Expanded Panel | CALR MPN Expanded Panel | JAK2 Exon 12 MPN Expanded Panel | JAK2 V617F Mutation Analysis | LAB3612 | 7135 | MPL MPN Expanded Panel

Interface Order Alias

55091

Quick Collect

+A

Clinical Information

The JAK2 V617F Mutation assay uses next generation sequencing to detect the presence of the V617F mutation which can be used to monitor disease progression in certain myeloproliferative disorders.  If the JAK2 V617F mutation is absent, the MPN Expanded panel will automatically reflex.

 

The MPN Expanded panel assay uses next generation sequencing and fragmentation analysis to detect well defined mutations in CALR exon 9, JAK2 exon 12 and MPL exon 10, which, in addition to BCR-ABL gene fusion and JAK2 V617F mutation testing, aids in the diagnosis of Myeloproliferative Neoplasms.

Specimen Requirements

Submit only one of the following specimens: (Blood or Bone Marrow)

Specimen Collection: Blood

Container(s): Lavender Top (EDTA)

Preferred Volume to Collect: 6.0 mL

Minimum Volume to Collect: 4.0 mL

Collection Instructions:

  • Gently invert EDTA tube(s) 8 - 10 times after collection.
  • For optimal results, the specimens should be received in the laboratory within 48 hours of collection

Processing Instructions (Laboratory, Outpatient or Off-site collection)

  • Processed Specimen: Whole Blood EDTA
  • Centrifuge/Spin: No
  • Aliquot: No
  • Transport Temperature: Ambient or Refrigerated

 

Specimen Collection: Bone Marrow

Container(s): Lavender Top (EDTA)

Preferred Volume to Collect: 6.0 mL

Minimum Volume to Collect: 4.0 mL

Collection Instructions:

  • For optimal results, the specimens should be received in the laboratory within 48 hours of collection

Processing Instructions (Laboratory, Outpatient or Off-site collection)

  • Transport Temperature: Ambient or Refrigerated

Specimen Stability

Ambient: 24 hours

Refrigerate: 72 hours

Laboratory Retention: Extracted DNA retained greater than 1 year

Reflex Information

Link: MEC Approved Reflex Testing 

Test Frequency

Available once per week, usual TAT less than 7 days

Reference Range

An interpretative report will be provided.

Performing Department

Molecular Diagnostics

Performing Department Laboratory Location

Corewell Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Next Generation Sequencing by Ion AmpliSeq technology and Fragmentation Analysis

CPT

81270, If test reflexes additional charges to follow see MPN Expanded Panel

CDM Code

3108127002

Epic Test ID

1230100853

Mayo Access Code

SHO55091

Reviewed Date

6/22/2023