Sign in →

Epic Code LAB3654 B-Acute Lymphocytic Leukemia (B-ALL) Probe Panel by FISH

Test Name Alias

B-ALL Panel | B-ALL Probe Panel by FISH | 7145 | LAB2111347

Interface Order Alias

55101

Quick Collect

AP Ambient

Clinical Information

This probe panel detects specific numerical and structural chromosome abnormalities commonly associated with B-ALL.

 

Routine chromosome analysis is performed on all diagnostic samples and is recommended on all bone marrow specimens to exclude abnormalities not identified by the specified FISH probe(s).

Panel Information

Panel testing includes:

  • 1q25 rearrangement, ABL2 break apart
  • +4/+10, D4Z1/D10Z1
  • 5q32 rearrangement, PDGFRB break apart
  • t(12;21), ETV6/RUNX1, iAMP21
  • 9p21, CDKN2A
  • 9q34 rearrangement, ABL1 break apart
  • t(9;22), BCR/ABL
  • 11q23 rearrangement, MLL (KMT2A)
  • 19p13 rearrangement, E2A break apart
    • Reflex: t(1;19), PBX/TCF3
       

If the standard B-ALL FISH panel demonstrates normal pattern for all probes OR if known Down Syndrome, reflex testing will be performed using CRLF2 break apart probe set to evaluate for t(Xp22.33;var) or t(Yp11.32;var).

Collection Instructions

Multiple specimen collection types. Submit only one:

 

Specimen Collection: Bone Marrow

Container(s): Green Top (Sodium Heparin)

Preferred volume to collect: 5.0 mL

Minimum volume to collect: 1.0 mL

Neonate volume to collect: 1.0 mL

Collection Instructions:

  • Collect bone marrow and transfer marrow from syringe to plain green top sodium heparin tube. 
  • A bone marrow sample is the preferred sample type for testing.

 

Specimen Collection: Peripheral blood
Container/Tube: Green Top (Sodium Heparin)

Preferred volume to collect: 8.0 mL

Minimum volume to collect: 0.5 mL

Neonate volume to collect: 0.5 mL

Collection Instructions:

  • Peripheral blood may be substituted for Bone Marrow if circulating blasts are present 
  • Collect leukemic peripheral blood into a plain green top sodium heparin tube. 
  • Keep sample at room temperature and send to the Lab immediately. 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Spin: No

Aliquot: No

 

Transport Temperature: Ambient

 

If ambient temperature exceeds 80°F or is below 40°F, please send in an insulated container. All samples should be directed to the laboratory as soon as possible.

Specimen Stability

Ambient: 3 days (beyond 3 days, specimen viability will be detemined by Cytogenetics Lab)

Laboratory Retention: primary specimens 4 weeks; frozen pellets retained greater than 1 year

Test Frequency

Test Set Up: Monday – Friday 8:00 am – 5:00 pm, Saturday 8:00 am – 3:00 pm.

Technologist on call for Sunday set up.

Final report: 2 – 3 days.

Reference Range

An interpretative report will be provided.

Performing Department

Cytogenetics

Performing Department Laboratory Location

Corewell Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Performed by Fluorescence In-Situ Hybridization (FISH). This test was developed and its performance characteristics determined by Spectrum Health Cytogenetics. It has not been cleared or approved by the U.S. FDA. The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes.

CPT

88271 x9, 88275 x18

CDM Code

3108827102, 3108827501

Epic Test ID

1230100291

LOINC

Specimen Type: 66746-9

Indication Provided: 42349-1

Result: in process

Interpretation: 59050-5

Comments: 69965-2

Reviewed Date

4/28/2023