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Epic Code LAB3654 B-Acute Lymphocytic Leukemia (B-ALL) Probe Panel by FISH

Test Name Alias

B-ALLPanel | B-ALL Probe Panel by FISH | 7145 | LAB2111347 | 1230100291

Interface Order Alias

55101

Quick Collect

AP Ambient

Clinical Information

This probe panel detects specific numerical and structural chromosome abnormalities commonly associated with B-ALL and includes automatic reflex to Philadelphia chromosome-like acute lymphoblastic leukemia (Ph-like ALL) testing.

 

Routine chromosome analysis is performed on all diagnostic samples and is recommended on all bone marrow specimens to exclude abnormalities not identified by the specified FISH probe(s).

Panel Information

Panel testing includes:

+4/+10, D4Z1/D10Z1

t(12;21), ETV6::RUNX1, iAMP21

t(9;22), BCR::ABL

11q23 rearrangement, MLL (KMT2A)

 

When initial testing is negative, the patient is known Down Syndrome, or of Hispanic descent, reflex testing includes:

t(Xp;var) or t(Yp;var), CRLF2 rearrangement.

 

When CRLF2 rearrangement is identified, reflex testing includes:

t(X;14) or t(Y;14) CRLF2::IGH fusion probe.

 

When CRLF2 rearrangement is not identified, reflex testing includes:

1q25 rearrangement, ABL2 break apart

5q32 rearrangement, PDGFRB break apart

9q34 rearrangement, ABL1 break apart

Collection Instructions

Multiple specimen collection types. Submit only one:

 

Specimen Collection: Bone Marrow

Container(s): Green Top (Sodium Heparin)

Preferred volume to collect: 5.0 mL

Minimum volume to collect: 1.0 mL

Neonate volume to collect: 1.0 mL

Collection Instructions:

  • Collect bone marrow and transfer marrow from syringe to plain green top sodium heparin tube. 
  • A bone marrow sample is the preferred sample type for testing.

 

Specimen Collection: Peripheral blood
Container/Tube: Green Top (Sodium Heparin)

Preferred volume to collect: 8.0 mL

Minimum volume to collect: 0.5 mL

Neonate volume to collect: 0.5 mL

Collection Instructions:

  • Peripheral blood may be substituted for Bone Marrow if circulating blasts are present 
  • Collect leukemic peripheral blood into a plain green top sodium heparin tube. 
  • Keep sample at room temperature and send to the Lab immediately. 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Spin: No

Aliquot: No

 

Transport Temperature: Ambient

 

If ambient temperature exceeds 80°F or is below 40°F, please send in an insulated container. All samples should be directed to the laboratory as soon as possible.

Specimen Stability

Ambient: 3 days (beyond 3 days, specimen viability will be detemined by Cytogenetics Lab)

Laboratory Retention: primary specimens 4 weeks; frozen pellets retained greater than 1 year

Test Frequency

Test Set Up: Monday – Friday 8:00 am – 5:00 pm, Saturday 8:00 am – 3:00 pm.

Technologist on call for Sunday set up.

Final report: 2 – 3 days.

Reference Range

An interpretative report will be provided.

Performing Department

Cytogenetics

Performing Department Laboratory Location

Corewell Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Performed by Fluorescence In-Situ Hybridization (FISH). This test was developed and its performance characteristics determined by Spectrum Health Cytogenetics. It has not been cleared or approved by the U.S. FDA. The FDA has determined that such clearance or approval is not necessary. This test is used for clinical purposes.

CPT

88271x4, 88275x8

88271x1, 88275x2 (each additional probe set)

CDM Code

3108827102, 3108827501

Epic Test ID

1230100291

LOINC

Specimen Type: 66746-9

Indication Provided: 42349-1

Result: in process

Interpretation: 59050-5

Comments: 69965-2

Reviewed Date

4/28/2023

Updated Date

7/3/2025 - Clinical Information, Panel Information, CPT

Orderable Regions

Southwest; West