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Epic Code LAB458 ANCA (Anti Neutrophil Cytoplasmic Antibody)

Test Name Alias

ANCA | cANCA | Wegener’s | Anticytoplasmic Autoantibodies | p ANCA | pANCA | 8556 | Granulomatosis 

Interface Order Alias

10089

Clinical Information

Includes c-ANCA (cytoplasmic) and p-ANCA (perinuclear)

Positive c-ANCA results are titered, with ≥1:20 considered positive

Collection Instructions

Specimen Collection: Blood

Container(s): Gold Top (Serum Separator-SST Gel)

Preferred Volume to Collect: 5.0 mL

Minimum Volume to Collect: 1.0 mL

Neonate Volume to Collect: 1.0 mL

Capillary collect ok: Yes

Microtainer acceptable: Yes

Collection Instructions:

  • After collection, gently invert tube 8-10 times.
  • Specimen should be processed within 2 hours – See Processing Instructions.

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Serum

Centrifuge/Spin: Yes

Aliquot: No

Processing Instructions:

  • Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.

Transport Temperature: Refrigerate

Rejection Criteria

Specimen not centrifuged within 2 hours

Specimen Stability

Ambient: 12 hours

Refrigerate: 7 days

Frozen: aliquot plasma/serum for longer storage

Laboratory Retention: 7 days

Test Frequency

Available Monday through Friday, usual TAT 1 to 3 days.

Reference Range

Negative < 1:20

Positive c-ANCA results are titered, with ≥1:20 considered positive

Performing Department

Immunochemistry

Performing Department Laboratory Location

Corewell Health Reference Laboratory, Grand Rapids, MI

Methodology

Immunofluorescence Assay (IFA)

CPT

86036

CDM Code

3028603601

Epic Test ID

1230100342

LOINC

c-ANCA: 35279-9

p-ANCA: 17357-5

p-ANCA Comment: 48767-8

Mayo Access Code

SHO8556

Reviewed Date

12/13/2022