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Epic Code LAB782 Fructosamine, Serum

Additional Codes

Mayo Code: FRUCT

Epic Code: LAB 782

Interface Order Alias: 10143

Cerner code: 8612

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Monitoring intermediate (1-3 weeks) glycemic control


Monitoring glycemic control in patients with shortened red blood cell survival

Specimen Type


Specimen Required

Supplies: Sarstedt 5 mL Aliquot Tube (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Serum gel tubes should be centrifuged within 2 hours of collection.

2. Red-top tubes should be centrifuged and the serum aliquoted into a plastic vial within 2 hours of collection.

Laboratory Test Directory Note:

COLLECTION NOTE: Volumes listed are in serum or plasma, draw approximately 2 1/2 times the requested volume in whole blood.

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 7 days
  Frozen  60 days
  Ambient  72 hours

Reject Due To

Gross hemolysis Reject
Gross icterus Reject

Day(s) Performed

Monday through Sunday

Reference Values

200-285 mcmol/L

Clinical Information

Fructosamine is a general term, which applies to any glycated protein. It is formed by the nonenzymatic reaction of glucose with the alpha- and epsilon-amino groups of proteins to form intermediate compounds called aldimines. These aldimines may dissociate or undergo an Amadori rearrangement to form stable ketoamines called fructosamines. This nonenzymatic glycation of specific proteins in vivo is proportional to the prevailing glucose concentration during the lifetime of the protein. Therefore, glycated protein measurement in a patient with diabetes is felt to be a better monitor of long-term glycemic control than individual or sporadic glucose determinations. The best known of these proteins is glycated hemoglobin, which is often measured as hemoglobin A1c, and reflects glycemic control over the past 6 to 8 weeks. In recognition of the need for a measurement that reflects intermediate-term glycemic control and was easily automated, a nonspecific test, termed fructosamine, was developed. Since albumin is the most abundant serum protein, it accounts for 80% of the glycated serum proteins, and thus, a high proportion of the fructosamine. Although a large portion of the color generated in the reaction is contributed by glycated albumin, the method will measure all proteins, each with a different half-life and different levels of glycation.


Since the assay is nonspecific, color may be generated by compounds other than glycated proteins. Interferences are seen from ascorbic acid (vitamin C) and elevated bilirubin values.


However, the second-generation assays have been shown to be highly specific for glycated proteins.


Fasting blood glucose and hemoglobin A1c are the usual and preferred means of monitoring glycemic control.


In general, fructosamine reflects glycemic control in diabetic patients over the previous 1 to 3 weeks. High values indicate poor control.


All glycated proteins are measured by this method, with glycated albumin contributing a large portion.

Reporting Name

Fructosamine, S

Method Name

Colorimetric Rate Reaction

Method Description

The fructosamine assay is a colorimetric test based on the ability of ketoamines to reduce nitroblue tetrazolium to formazan in an alkaline medium. The rate of formation of formazan is directly proportional to the concentration of fructosamine, and is measured photometrically at 546 nm.(Package insert: Fructosamine reagent. Roche Diagnostics; v. 8.0, 12/2018)

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
FRUCT Fructosamine, S 15069-8


Result ID Test Result Name Result LOINC Value
FRUCT Fructosamine, S 15069-8

Report Available

Same day/1 to 2 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

Clinical Reference

1. Sacks DB: Diabetes mellitus. In: Rifai N, Horvath AR, Wittwer CT, eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 6th ed. Elsevier; 2018:1194-1197

2. Masharani U, German MS: Pancreatic hormones and diabetes mellitus. In: Gardner DG, Shoback D, eds. Greenspan’s Basic and Clinical Endocrinology. 9th ed. McGraw; 2018:46-47

3. Austin GE, Mullins RH, Morin LG: Non-enzymatic glycation of individual plasma proteins in normoglycemic and hyperglycemic patients. Clin Chem. 1987;33:2220-2224

4. Schleicher ED, Mayer R, Wagner EM, Gerbitz KD: Is serum fructosamine assay specific for determination of glycated serum protein? Clin Chem. 1988;34:320-323