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Epic Code LAB786 Granulocyte Antibodies, Serum

Additional Codes

Mayo Code: LAGGT

Interface Order Alias: 10391

Cerner: 8607

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Useful For

Work-up of individuals having febrile, nonhemolytic transfusion reactions

 

Detection of individuals with autoimmune neutropenia

 

This test is not useful for the diagnosis of neutropenia caused by marrow suppression by drugs or tumors.

Specimen Type

Serum Red
Laboratory Test Directory Note:

COLLECTION NOTE: Volumes listed are in serum or plasma, draw approximately 2 1/2 times the requested volume in whole blood.


Specimen Required


Collection Container/Tube: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.

Additional Information: Only pretransfusion reaction specimen is acceptable.


Laboratory Test Directory Note:

COLLECTION NOTE: Volumes listed are in serum or plasma, draw approximately 2 1/2 times the requested volume in whole blood.

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Red Refrigerated (preferred) 30 days
  Frozen  365 days
  Ambient  7 days

Reject Due To

Gross hemolysis OK
Gross lipemia OK
Gross icterus OK

Day(s) Performed

Tuesday, Wednesday, Friday

Reference Values

Not applicable

Clinical Information

Granulocyte antibodies are induced by pregnancy or prior transfusion and are associated with febrile, nonhemolytic transfusion reactions. Patients who have been immunized by previous transfusions, pregnancies, or allografts frequently experience febrile, nonhemolytic transfusion reactions that must be distinguished from hemolysis before further transfusions can be safely administered. Granulocyte antibodies may also be present in autoimmune neutropenia.

Cautions

No significant cautionary statements

Interpretation

A positive result in an individual being worked up for a febrile transfusion reaction indicates the need for leukocyte-poor (filtered) red blood cells.

 

This test cannot distinguish between allo- and autoantibodies

Reporting Name

Granulocyte Ab, S

Method Name

Indirect Immunofluorescence

Method Description

Purified granulocyte preparations from normal donors are incubated with patient's test serum and then with fluorescein-tagged antihuman globulin reagent. Sera containing the antibodies deposit immunoglobulin on the target cell membrane, which is detected by the second stage antibody and visualized by fluorescence microscopy.(Verheugt FW, von dem Borne AE, Decary F, Engelfreit CP. The detection of granulocyte alloantibodies with an indirect immunofluorescence test. Br J Haematol. 1997;36(4):533-534)

CPT Code Information

86021

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LAGGT Granulocyte Ab, S 35279-9

 

Result ID Test Result Name Result LOINC Value
LAGG Granulocyte Ab, S 35279-9

Report Available

7 to 15 days

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

Clinical Reference

1. Flesch BK, Reil A. Molecular genetics of the human neutrophil antigens. Transfus Med Hemother. 2018;45(5):300-309. doi:10.1159/000491031

2. Gottschall JL, Triulzi DJ, Curtis B, et al. The frequency and specificity of human neutrophil antigen antibodies in a blood donor population. Transfusion. 2011;51(4):820-827. doi:10.1111/j.1537-2995.2010.02913.x