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Epic Code LAB838 Pentobarbital Level

Test Name Alias

Pentobarbital Blood Level | Nembutal | 8290

Interface Order Alias

10448

Collection Instructions

Specimen Collection: Blood

 

Container(s): Gray Top (Potassium Oxalate and Sodium Fluoride) or Red top (no additive)

Preferred Volume to Collect: 6.0 mL

Minimum Volume to Collect: 4.0 mL

Neonate Volume to Collect: 4.0 mL

Capillary collect ok? Yes

Microtainer acceptable?  Yes

 

Collection Instructions:

  • After collection, gently invert tube 8-10 times.
  • Gray Top is preferred tube.
  • Specimen should be processed – See Processing Instructions.

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Plasma or Serum

Centrifuge/Spin: Yes

Aliquot: Yes

 

Processing Instructions:

  • Gray top:
    • Centrifuge after collection
    • Pipette plasma into aliquot tubes
  • Red top:
    • When tubes are clotted (30-45 minutes), centrifuge specimens
    • Pipette serum into aliquot tube

Minimum plasma/serum volume: 0.5 mL

 

Transport Temperature: Ambient

Specimen Stability

Ambient: 14 days

Refrigerate: 14 days

Frozen: 14 days

Laboratory Retention: 7 days

Test Frequency

Available 24 hours, usual TAT 1 day.

STAT TAT 4 hours.

Reference Range

  1.  
  • Sedation 1 to 5 µg/mL
  • Therapeutic coma 20 to 50 µg/mL
  • Reducing Intracranial Pressure 30 to 40 µg/mL

Performing Department

Toxicology

Performing Department Laboratory Location

Corewell Health Reference Laboratory, Grand Rapids, MI

Methodology

Liquid Chromatography-Mass Spectrometry

 

This test was developed and its performance characteristics determined by Spectrum Health Toxicology Laboratory. It has not been cleared or approved by the FDA. The laboratory is regulated under CLIA as qualified to perform high-complexity testing. This test is used for clinical purposes. It should not be regarded as investigational or for research.

 

CPT

80345, G0480

CDM Code

3018034502

Epic Test ID

1230101030

LOINC

Pentobarbital Lvl: 3924-8

Reviewed Date

2/15/2023