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Mayo Clinic Laboratories

Epic Code LAB844 Protein Electrophoresis, Serum, IFE if Indicated

Test Name Alias

Protein Electrophoresis IFE if Indicated | SPEP | S-PEP | Immunofixation | 8306

Interface Order Alias

10811

Clinical Information

Test will include serum protein and pathologist interpretation. For guidance on ordering protein and urine electrophoresis, please refer to Orders- Protein Orderable Changes (link). 

Ordering Instructions

Protein Electrophoresis cannot be ordered a second time within a 2 week window in Laboratory Information System (LIS)

 

Collection Instructions

Specimen Collection: Blood

Container(s): Gold Top (Serum Separator-SST Gel)

Preferred Volume to Collect: 5.0 mL

Minimum Volume to Collect: 2.0 mL

Neonate Volume to Collect: 2.0 mL

Capillary collect ok: Yes

Microtainer acceptable: Yes

Collection Instructions:

  • Fasting specimen preferred, but not required.
  • Gently invert gold tube(s) 8 - 10 times to mix clot activator with blood.
  • Specimen should be processed within 2 hours – See Processing Instructions.

 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

 

Processed Specimen: Serum

Centrifuge/Spin: Yes

Aliquot: No

Processing Instructions:

  • Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.

Transport Temperature: Refrigerate

Rejection Criteria

Specimen not centrifuged within 2 hours

Specimen Stability

Ambient: 8 hours

Refrigerate: 7 days

Laboratory Retention: 7 days

Reflex Information

Reflex approved by Medical Executive Committee. Please see link: Reflex Testing Document

Test Frequency

Available Monday – Friday, TAT up to 5 business days depending on if the specimen needs additional testing.

Reference Range

Protein total

0 up to 8 Days: 4.5 – 7.0 g/dL
8 Days up to 3 Years: 5.5 – 7.5 g/dL
>3 Years: 6.0 – 8.0 g/dL

 

Albumin SPE: 3.7 – 4.9 g/dL

 

Alpha 1 Globulin: 0.2 – 0.4 g/dL

 

Alpha 2 Globulin: 0.5 – 0.9 g/dL

 

Beta Globulin:  0.6 – 1.0 g/dL

 

Gamma Globulin: 0.6 – 1.4 g/dL

 

 

Performing Department

Multiple Departments: Chemistry and Immunochemistry

Performing Department Laboratory Location

Corewell Health Reference Laboratory, Grand Rapids, MI

Methodology

Capillary Zone Electrophoresis

CPT

84165 x 2 for Serum protein electrophoresis : Technical and Professional billing
86334 x 2 for reflex Serum immunofixation: Technical and Professional billing

CDM Code

3018416501, 3018416501, 84165, 84165

Epic Test ID

1230101084

Mayo Access Code

SHO8306

Reviewed Date

3/24/2025