Epic Code LAB844 Protein Electrophoresis, Serum, IFE if Indicated
Test Name Alias
Protein Electrophoresis IFE if Indicated | SPEP | S-PEP | Immunofixation | 8306
Interface Order Alias
10811
Clinical Information
Test will include serum protein and pathologist interpretation. For guidance on ordering protein and urine electrophoresis, please refer to Orders- Protein Orderable Changes (link).
Ordering Instructions
Protein Electrophoresis cannot be ordered a second time within a 2 week window in Laboratory Information System (LIS)
Collection Instructions
Specimen Collection: Blood
Container(s): Gold Top (Serum Separator-SST Gel)
Preferred Volume to Collect: 5.0 mL
Minimum Volume to Collect: 2.0 mL
Neonate Volume to Collect: 2.0 mL
Capillary collect ok: Yes
Microtainer acceptable: Yes
Collection Instructions:
- Fasting specimen preferred, but not required.
- Gently invert gold tube(s) 8 - 10 times to mix clot activator with blood.
- Specimen should be processed within 2 hours – See Processing Instructions.
Processing Instructions (Laboratory, Outpatient or Off-site collection)
Processed Specimen: Serum
Centrifuge/Spin: Yes
Aliquot: No
Processing Instructions:
- Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.
Transport Temperature: Refrigerate
Rejection Criteria
Specimen not centrifuged within 2 hours
Specimen Stability
Ambient: 8 hours
Refrigerate: 7 days
Laboratory Retention: 7 days
Reflex Information
Reflex approved by Medical Executive Committee. Please see link: Reflex Testing Document
Test Frequency
Available Monday – Friday, TAT up to 5 business days depending on if the specimen needs additional testing.
Reference Range
Protein total
0 up to 8 Days: 4.5 – 7.0 g/dL
8 Days up to 3 Years: 5.5 – 7.5 g/dL
>3 Years: 6.0 – 8.0 g/dL
Albumin SPE: 3.7 – 4.9 g/dL
Alpha 1 Globulin: 0.2 – 0.4 g/dL
Alpha 2 Globulin: 0.5 – 0.9 g/dL
Beta Globulin: 0.6 – 1.0 g/dL
Gamma Globulin: 0.6 – 1.4 g/dL
Performing Department
Multiple Departments: Chemistry and Immunochemistry
Performing Department Laboratory Location
Corewell Health Reference Laboratory, Grand Rapids, MI
Methodology
Capillary Zone Electrophoresis
CPT
84165 x 2 for Serum protein electrophoresis : Technical and Professional billing
86334 x 2 for reflex Serum immunofixation: Technical and Professional billing
CDM Code
3018416501, 3018416501, 84165, 84165
Epic Test ID
1230101084
Mayo Access Code
SHO8306
Reviewed Date
3/24/2025