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HelpEpic Code LAB848 Hepatitis C Virus (HCV) 1a NS5A Drug Resistance by Sequencing
Test Name Alias
Hepatitis C Virus | HCV | 7142 | Hepatitis C 1a NS5A Resistance-Associated Variants (RAVs)
Clinical Information
Order before initiating treatment with NS5A inhibitors. Do not order prior to molecular confirmation of positive hepatitis C virus (HCV) screen and confirmation of genotype 1a or 1b.
This assay detects resistance-associated variants in NS5A codons 20-101 for HCV genotypes 1a and 1b. Variants in viral sub-populations below 20 percent of total may not be detected. For further information, please refer to drug package inserts for the applicable direct-acting antiviral drug and current HCV treatment guidelines (eg., AASLD/IDSA guidelines or EASL HCV treatment recommendations).
This test may be unsuccessful if the HCV RNA viral load is less than log 3.4 or 2500 IU/mL and/or if the HCV RNA genotype is not 1a or 1b.
Specimen Requirements
Specimen Requirements:
Preferred
Specimen Type: Plasma
Container Tube: Lavender top (EDTA)
Volume: 6 mL whole blood collected in EDTA tube(s)
Minimum volume: 2 mL plasma
Collection Instructions: Centrifuge blood in EDTA tube within 2 hours of collection and transfer plasma to a screw-capped transport tube.
Acceptable
Specimen Type: Serum
Container Tube: Gold Top (Serum Separator-SST Gel)
Volume: 5 mL whole blood collected in Gold Top tube
Minimum volume: 2 mL serum
Collection Instructions: Centrifuge blood in Gold Top tube within 2 hours of collection and transfer serum to a screw-capped transport tube.
Specimen Transport Temperature
Frozen
Specimen Stability
Ambient: 24 hours
Refrigerate: 3 days
Frozen: 6 weeks
Laboratory Retention: 2 months
Test Frequency
Reported: 10-13 days
Performing Department
REFERRALS SEND OUTS
Performing Department Laboratory Location
Send Out to ARUP Laboratory
Methodology
Polymerase Chain Reaction/Sequencing
Reviewed Date
6/9/2023