Sign in →

Epic Code LAB9510 Hepatitis B Virus DNA Quantitation by PCR

Test Name Alias

Hepatitis B Virus DNA Quantitative | 230

Interface Order Alias

10583

Quick Collect

+S/PO/F

Collection Instructions

Specimen Collection: Blood

 

Container(s): 6 mL Pink Top (EDTA) OR 5 mL Gold top (Serum Separator-SST Gel)

Preferred Volume to Collect: Full tube (6 mL or 5 mL)

 

Collection Instructions:

  • Gently invert EDTA tube(s) 8 - 10 times after collection.
  • Specimen should be processed within 24 hours – See Processing Instructions.

 

Processing Instructions (Laboratory, Outpatient or Off-site collection)

  • Processed Specimen: Plasma or Serum
  • Centrifuge/Spin: Yes
  • Aliquot: Yes
  • Processed Minimum Volume: 2.0 mL Plasma or Serum
  • Processing Instructions:
    • Plasma is preferred specimen type
    • With room temperature storage, centrifuge whole blood in EDTA or Serum Separator tube within 24 hours of collection. 
    • With refrigerated temperature storage, centrifuge whole blood in EDTA or Serum Separator tube within 72 hours of collection. 
    • Transfer plasma or serum to a screw-capped transport tube.
    • Plasma or serum needs to be stored frozen in screw-capped transport tube after separation from cells
  • Transport temperature: Prefer that plasma or serum be frozen for transport

 

Specimen Stability

Ambient: 24 hours

Refrigerate: 3 days

Frozen: 60 days

Laboratory Retention:1 month

Test Frequency

Test performed weekly, usual TAT less than 7 days

Reference Range

HBV Viral Load

Not Detected: <10 IU/mL

 

Linear range of positive results

Positive: 10 - 1,000,000,000 (1e9) IU/mL

Equivocal: Detected but below lower limit of quantitation (<10 IU/mL)

Performing Department

Molecular Diagnostics

Performing Department Laboratory Location

Corewell Health Advanced Technology Laboratory (ATL), Grand Rapids, MI

Methodology

Quantitative Real-Time Polymerase Chain Reaction (RT-PCR)

CPT

87517

CDM Code

3068751701

Epic Test ID

1230100761

Mayo Access Code

SHO230

Reviewed Date

6/22/2023