Epic Code LAB9510 Hepatitis B Virus DNA Quantitation by PCR
Test Name Alias
Hepatitis B Virus DNA Quantitative | 230
Interface Order Alias
10583
Quick Collect
+S/PO/F
Collection Instructions
Specimen Collection: Blood
Container(s): 6 mL Pink Top (EDTA) OR 5 mL Gold top (Serum Separator-SST Gel)
Preferred Volume to Collect: Full tube (6 mL or 5 mL)
Collection Instructions:
- Gently invert EDTA tube(s) 8 - 10 times after collection.
- Specimen should be processed within 24 hours – See Processing Instructions.
Processing Instructions (Laboratory, Outpatient or Off-site collection)
- Processed Specimen: Plasma or Serum
- Centrifuge/Spin: Yes
- Aliquot: Yes
- Processed Minimum Volume: 2.0 mL Plasma or Serum
- Processing Instructions:
- Plasma is preferred specimen type
- With room temperature storage, centrifuge whole blood in EDTA or Serum Separator tube within 24 hours of collection.
- With refrigerated temperature storage, centrifuge whole blood in EDTA or Serum Separator tube within 72 hours of collection.
- Transfer plasma or serum to a screw-capped transport tube.
- Plasma or serum needs to be stored frozen in screw-capped transport tube after separation from cells
- Transport temperature: Prefer that plasma or serum be frozen for transport
Specimen Stability
Ambient: 24 hours
Refrigerate: 3 days
Frozen: 60 days
Laboratory Retention:1 month
Test Frequency
Test performed weekly, usual TAT less than 7 days
Reference Range
HBV Viral Load
Not Detected: <10 IU/mL
Linear range of positive results
Positive: 10 - 1,000,000,000 (1e9) IU/mL
Equivocal: Detected but below lower limit of quantitation (<10 IU/mL)
Performing Department
Molecular Diagnostics
Performing Department Laboratory Location
Corewell Health Advanced Technology Laboratory (ATL), Grand Rapids, MI
Methodology
Quantitative Real-Time Polymerase Chain Reaction (RT-PCR)
CPT
87517
CDM Code
3068751701
Epic Test ID
1230100761
Mayo Access Code
SHO230
Reviewed Date
6/22/2023