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Epic Code TropI Gene Therapy High Sensitivity Troponin I (specific use only)

Important Note

  • This testing is only available to approved Corewell Health providers in West Michigan. 
    • To be collected at hospital sites only. In Grand Rapids, outpatients may only be collected at the 35 Michigan Draw Site

Ordering Instructions

This testing is only available to approved ordering providers in West Michigan.

This testing may only be collected Monday to Thursday or early Friday morning to avoid missing the Friday 2 p.m. courier.

Specimen Type

Blood

Collection Instructions

  • One Pearl-top K2EDTA PPT plasma gel separator tube (BD 362788) or lavender K2EDTA plasma tube. (Minimum Whole Blood: 4.0 mL) Do NOT use any other blood tube or microtainer. 
  • Invert tube 8 times for proper mixing of EDTA anticoagulant, then centrifuge immediately to separate plasma from cells, 1300 x g for 10 minutes (e.g., Drucker horizon mini E centrifuge).
  • Refrigerate (2-8°C or 36-46°F) the centrifuged tube. (Minimum: 1.0 mL plasma) 
  • Centrifuged Pearl-top or lavender-top tube, refrigerated (28°C or 36-46°F). (Minimum: 1.0 mL plasma)
  • Labeled aliquot tube, refrigerated (2-8°C or 36-46°F). (Minimum: 1.0 mL plasma) 

 

In-Lab Processing

  • Centrifuge Pearl-top or lavender top tubes received from inpatient locations to separate plasma from cells.
  • For STAT troponin orders, centrifuge at 4000 x g for 3 minutes (e.g., StatSpin Express 4 centrifuge).
  • For routine analysis, centrifuge at 1300 x g for 5 minutes.
  • Specimens should be free of particulate matter.
  • Deliver immediately to the appropriate testing station or add aliquoted labeled plasma sample to the automated line. 

Rejection Criteria

  • Pearl-top or lavender top tubes with plasma not separated from cells within eight hours of collection.
  • Specimens collected in inappropriate collection tubes. 
  • Specimens collected in microtainer tubes.
  • Specimens not collected and processed as indicated.
  • Grossly hemolyzed specimens. 

Specimen Stability

Pearl-top K2EDTA plasma gel separator tube OR spun-down plasma aliquot

  • Room Temperature (20-26° C or 68-78.8°F): 24 hours
  • Refrigerated (2-8°C or 36-46°F): 5 days
  • Frozen (-20°C/-4°F): Unacceptable

Lavender K2EDTA tube (Centrifuged and NOT Centrifuged)

  • Room Temperature (20-26° C or 68-78.8°F): 8 hours
  • Refrigerated (2-8°C or 36-46°F): 8 hours
  • Frozen (-20°C/-4°F): Unacceptable

Reference Range

< 4 ng/L for single-Troponin rule-out algorithm. 

Female:   ≤ 17 ng/L  (≤ 99th Percentile)
Male:       ≤ 35 ng/L  (≤ 99th Percentile) 

See attached algorithm for significant 2-hour delta Troponin values. 

Performing Department Laboratory Location

Royal Oak Automated Chemistry Laboratory 

Methodology

Automated Chemiluminescence Immunoassay.

CPT

84484

CDM Code

3008448401

Reviewed Date

10/29/2024