Monoclonal Gammopathy Screen - Laboratory Test Directory

Test Name Alias

Protein Electrophoresis IFE if Indicated | SPEP | Immunofixation | Kappa and Lambda serum free light chains

Interface Order Alias

1230573

Clinical Information

The Monoclonal Gammopathy Screen reflects expert recommendations for the first line testing for monoclonal gammopathies (plasma cell proliferative disorders). If amyloidosis (AL) is suspected, first line testing should also include Protein Electrophoresis, 24 Hour Urine, do IFE if Indicated (LAB2111444). For guidance on ordering protein and urine electrophoresis, please refer to Orders- Protein Orderable Changes.

Panel Information

This panel includes:

Ordering Instructions

Protein Electrophoresis cannot be ordered a second time within a 2 week window in Laboratory Information System (LIS)

Collection Instructions

Specimen Collection: Blood

Container(s): Gold Top (Serum Separator-SST Gel)

Preferred Volume to Collect: 5.0 mL
Minimum Volume to Collect: 2.0 mL
Neonate Volume to Collect: 2.0 mL
Capillary collect ok: Yes
Microtainer acceptable: Yes

Collection Instructions:

Fasting specimen preferred but not required.
Gently invert gold tube(s) 8 - 10 times to mix clot activator with blood.
Specimen should be processed within 2 hours – See Processing Instructions.

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Serum

Centrifuge/Spin: Yes

Aliquot: No

Processing Instructions:

Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.

Transport Temperature: Refrigerate

Rejection Criteria

Specimen not processed within 2 hours
Protein Electrophoresis cannot be ordered a second time within a 2 week window in the Laboratory Information System (LIS)

Specimen Stability

Ambient: 8 hours

Refrigerate: 7 days

Laboratory Retention: 7 days

Reflex Information

Reflex approved by Medical Executive Committee. Please see link: Reflex Testing Document

Test Frequency

Monday through Friday

Turn-around time: 1-3 days

Reference Range

Kappa and Lambda Free Light Chains

Kappa Free Light Chain: ≥18 Years: 0.33 – 1.94 mg/dL

Lambda Free Light Chain: ≥18 Years: 0.57 – 2.63 mg/dL

Kappa Lambda FLC Ratio: ≥18 Years: 0.26 – 1.65 mg/dL

Serum Protein Electrophoresis

Total Protein

0 up to 8 Days: 4.5 – 7.0 g/dL

8 Days up to 3 Years: 5.5 – 7.5 g/dL

>3 Years: 6.0 – 8.0 g/dL

Protein Fractions

Albumin: 3.7 – 4.9 g/dL

Alpha 1 Globulin: 0.2 – 0.4 g/dL

Alpha 2 Globulin: 0.5 – 0.9 g/dL

Beta Globulin: 0.6 – 1.0 g/dL

Gamma Globulin: 0.6 – 1.4 g/dL

Performing Department

Immunochemistry

Performing Department Laboratory Location

Corewell Health Reference Laboratory, Grand Rapids, MI

Methodology

See individual tests for Methodology

CPT

83883 x2
84165
Professional fee for pathologist interpretation

CDM Code

Unavailable

Epic Test ID

1230100856, 1230101084, 1230101084

LOINC

Unknown

Mayo Access Code