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Epic Code LAB1230573 Monoclonal Gammopathy Screen

Test Name Alias

Protein Electrophoresis IFE if Indicated | SPEP | Immunofixation | Kappa and Lambda serum free light chains

Interface Order Alias


Clinical Information

The Monoclonal Gammopathy Screen reflects expert recommendations for the first line testing for monoclonal gammopathies (plasma cell proliferative disorders).  If amyloidosis (AL) is suspected, first line testing should also include Protein Electrophoresis, 24 Hour Urine, do IFE if Indicated (LAB2111444). For guidance on ordering protein and urine electrophoresis, please refer to Orders- Protein Orderable Changes


Ordering Instructions

Protein Electrophoresis cannot be ordered a second time within a 2 week window in Laboratory Information System (LIS)


Collection Instructions

Specimen Collection: Blood

Container(s): Gold Top (Serum Separator-SST Gel)

  • Preferred Volume to Collect: 5.0 mL
  • Minimum Volume to Collect: 2.0 mL
  • Neonate Volume to Collect: 2.0 mL
  • Capillary collect ok: Yes
  • Microtainer acceptable: Yes

Collection Instructions:

  • Fasting specimen preferred but not required.
  • Gently invert gold tube(s) 8 - 10 times to mix clot activator with blood.
  • Specimen should be processed within 2 hours – See Processing Instructions.


Processing Instructions (Laboratory, Outpatient or Off-site collection)


Processed Specimen: Serum

Centrifuge/Spin: Yes

Aliquot: No

Processing Instructions:

  • Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.

Transport Temperature: Refrigerate

Rejection Criteria

  • Specimen not processed within 2 hours
  • Protein Electrophoresis cannot be ordered a second time within a 2 week window in the Laboratory Information System (LIS)

Specimen Stability

Ambient: 8 hours

Refrigerate: 7 days

Laboratory Retention: 7 days

Reflex Information

Reflex approved by Medical Executive Committee. Please see link: Reflex Testing Document

Test Frequency

Monday through Friday

Turn-around time: 1-3 days

Reference Range

Kappa and Lambda Free Light Chains

Kappa Free Light Chain: ≥18 Years: 0.33 – 1.94 mg/dL

Lambda Free Light Chain: ≥18 Years: 0.57 – 2.63 mg/dL

Kappa Lambda FLC Ratio: ≥18 Years: 0.26 – 1.65 mg/dL


Serum Protein Electrophoresis

Total Protein

0 up to 8 Days: 4.5 – 7.0 g/dL

8 Days up to 3 Years: 5.5 – 7.5 g/dL

>3 Years: 6.0 – 8.0 g/dL

Protein Fractions

Albumin: 3.7 – 4.9 g/dL

Alpha 1 Globulin: 0.2 – 0.4 g/dL

Alpha 2 Globulin: 0.5 – 0.9 g/dL

Beta Globulin: 0.6 – 1.0 g/dL

Gamma Globulin: 0.6 – 1.4 g/dL

Performing Department


Performing Department Laboratory Location

Corewell Health Reference Laboratory, Grand Rapids, MI


See individual tests for Methodology


83883 x2
Professional fee for pathologist interpretation

CDM Code


Epic Test ID

1230100856, 1230101084, 1230101084



Mayo Access Code


Reviewed Date