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Epic Code LAB3534 ACTH Level

Important Note

Only use pre-cooled sample tubes. Morning collection (6 am - 10 am) is optimal.

Method is Roche cobas Electrochemiluminescense Immunoassay.
Patient results determined by assays using different manufacturers for methods may not be comparable.

Test Name Alias

Adrenocorticotropic Hormone | 6662 | 1230100301

Interface Order Alias

11788

Quick Collect

PCT/ICED+(1)S/PO/F

Clinical Information

Useful for determining the cause of hypercortisolism and hypocortisolism

Ordering Instructions

  • Routine/Random Cortisol does not need to be scheduled. 
  • Routine/Random ACTH testing does not need to be scheduled.
  • ACTH Stimulation testing must be scheduled with a Corewell Health Infusion Clinic. Corewell Health Outpatient Phlebotomy sites do not provide ACTH Stimulation testing. 

Icons & Photos

Special handling: follow collection instructions below

Collection Instructions

Specimen Collection: Blood

 

Container(s): Pre-Cooled, Plastic Lavender EDTA

Preferred Volume to Collect: 3.0 mL

Minimum Volume to Collect: 1.0 mL

Neonate Volume to Collect: 1.0 mL

Capillary collect ok? No

 

Collection Instructions:

  • PCT: Only use pre-chilled tubes
  • Morning collection is optimal (6 am - 10 am)
  • ON ICE: After collection immediately put specimen on ice
  • Specimen should be processed immediately.

 

Processing Instructions (Laboratory, Outpatient or Off-site Collection):

Processed Specimen: Plasma (0.5 mL)

Centrifuge/Spin: Yes*

Aliquot: Yes

Processing Instructions:

  • +(1)S/PO/F: Within one hour of collection, spin at 3500 for 5 minutes* or 3300 for 10 minutes in a prechilled red holder
  • *Use refrigerated centrifuge to separate the plasma.
  • Aliquot and freeze immediately.

Transport Temperature: Frozen

Specimen Stability

Refrigerated (separated plasma): 3 hours

Frozen: 4 weeks

Laboratory Retention: 72 hours

Test Frequency

Available Monday, Wednesday & Friday, TAT 24 hours

Reference Range

10 - 60 pg/mL (a.m. draws) No established reference values for p.m. draws. 

Critical Values Table (for ages 0 - 18 years)

Performing Department

Chemistry

Performing Department Laboratory Location

Corewell Health Reference Laboratory, Grand Rapids, MI

Methodology

Method is Roche cobas Electrochemiluminescense Immunoassay.
Patient results determined by assays using different manufacturers for methods may not be comparable.

CPT

82024

CDM Code

3018202401

Epic Test ID

1230100301

LOINC

Not available

Reviewed Date

3/7/2024

Updated Date

5/27/2025 - ACTH Stimulation testing updated link

Orderable Regions

Southwest; West