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Mayo Clinic Laboratories

Epic Code LAB61 Cortisol

Important Note

Link: ACTH Stimulation Testing must be scheduled. See Ordering Instructions below.

Link: Adrenal Vein Specimen Instructions

Interface Order Alias

10113

Ordering Instructions

Note: Routine Cortisol does not need to be scheduled.

 

Grand Rapids: For scheduling ACTH Stimulation testing, please call the Spectrum Health Infusion Clinic at 616-486-6099 in Grand Rapids.

 

United Hospital: For scheduling ACTH Stimulation testing, contact the Cancer Center at 616-225-9330.

Collection Instructions

Specimen Collection: Blood

 

Container(s): 4.5 mL Mint Green Top (Lithium Heparin Gel) or 5.0 mL Gold Top (Serum Separator-SST Gel)

Preferred Volume to Collect: 4.5 mL

Minimum Volume to Collect: 1.0 mL

Neonate Volume to Collect: 0.5 mL

Capillary collect ok? Y

Microtainer acceptable: Y

 

Collection Instructions:

  • Indicate draw time on specimen.
  • After collection, gently invert tube 8-10 times.
  • Specimen should be processed within 2 hours – See Processing Instructions.

 

Processing Instructions (Laboratory, Outpatient or Off-site collection) 

 

Processed Specimen: Plasma or Serum

Centrifuge/Spin: Yes

Aliquot: Not needed

Processing Instructions:

  • Plasma is preferred specimen type
  • Green Tube/Plasma: Centrifuge after collection.
  • Gold Tube/Serum: Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.

Transport Temperature: Refrigerate

Specimen Transport Temperature

Refrigerated

Specimen Stability

Ambient: 12 hours

Refrigerated: 72 hours

Frozen: aliquot plasma/serum for longer storage

Laboratory Retention: 72 hours

Test Frequency

Available daily, usual TAT 1 day.

Reference Range

CHW:

  • A.M.: 6.0 – 22.0 µg/dL
  • P.M.: 3.0 – 14.0 µg/dL

 

CHS:

  • Morning: 6.0-18.4 µg/dL
  • Afternoon: 2.7-10.5 µg/dL
  • Post-Stimulation: >20.0 µg/dL

Performing Department

Chemistry

Performing Department Laboratory Location

CHW:

Corewell Health Reference Laboratory, Grand Rapids, MI
Corewell Health Blodgett Laboratory, Grand Rapids, MI

CHS:
Corewell Health Lakeland St. Joseph Laboratory, St. Joseph, MI

Methodology

Electrochemiluminescence Immunoassay (ECLIA)

CPT

82533

CDM Code

3018253302

Epic Test ID

1230100523

LOINC

Cortisol Lvl: 2143-6

Mayo Access Code

SHO6520

Reviewed Date

3/7/2024

Updated Date

3/14/2024 - Reference Range

5/2/2025 - CHS info added